Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 320013 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#320013, RRID:AB_439749
- Product name
- Alexa Fluor® 647 anti-mouse/rat/human FOXP3 [150D]
- Antibody type
- Monoclonal
- Antigen
- full-length FOXP3 protein
- Description
- FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 25 tests
- Storage
- The FOXP3 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references Therapeutic glucocorticoid-induced TNF receptor-mediated amplification of CD4+ T cell responses enhances antiparasitic immunity.
Therapy of experimental type 1 diabetes by isolated Sertoli cell xenografts alone.
Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells.
The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses.
Deficient CD4+CD25high T regulatory cell function in patients with active systemic lupus erythematosus.
Haque A, Stanley AC, Amante FH, Rivera Fde L, Zhou Y, Kuns RD, Yardley V, Sakaguchi S, Hill GR, Engwerda CR
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2583-92
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2583-92
Therapy of experimental type 1 diabetes by isolated Sertoli cell xenografts alone.
Fallarino F, Luca G, Calvitti M, Mancuso F, Nastruzzi C, Fioretti MC, Grohmann U, Becchetti E, Burgevin A, Kratzer R, van Endert P, Boon L, Puccetti P, Calafiore R
The Journal of experimental medicine 2009 Oct 26;206(11):2511-26
The Journal of experimental medicine 2009 Oct 26;206(11):2511-26
Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells.
Klunker S, Chong MM, Mantel PY, Palomares O, Bassin C, Ziegler M, Rückert B, Meiler F, Akdis M, Littman DR, Akdis CA
The Journal of experimental medicine 2009 Nov 23;206(12):2701-15
The Journal of experimental medicine 2009 Nov 23;206(12):2701-15
The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses.
Jordan JM, Woods ME, Olano J, Walker DH
Infection and immunity 2008 Aug;76(8):3717-24
Infection and immunity 2008 Aug;76(8):3717-24
Deficient CD4+CD25high T regulatory cell function in patients with active systemic lupus erythematosus.
Valencia X, Yarboro C, Illei G, Lipsky PE
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2579-88
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2579-88
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- C57BL/6 splenocytes surface stained with CD4 FITC, then intracellular stained with 150D Alexa Fluor® 647. Quadrant markers were set based on staining with Alexa Fluor® 647 mouse IgG1, κ isotype control
- Conjugate
- Red dye