Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 320101 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#320101, RRID:AB_430880
- Product name
- Purified anti-human FOXP3
- Antibody type
- Monoclonal
- Antigen
- full-length FOXP3 protein
- Description
- FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
Submitted references Expansion of FOXP3+ CD8 T cells with suppressive potential in colorectal mucosa following a pathogenic simian immunodeficiency virus infection correlates with diminished antiviral T cell response and viral control.
Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function.
Validated protocol for FoxP3 reveals increased expression in type 1 diabetes patients.
Evidence of systemic Th2-driven chronic inflammation in patients with metastatic melanoma.
FOXP3 expression in blood, synovial fluid and synovial tissue during inflammatory arthritis and intra-articular corticosteroid treatment.
Idiopathic CD4+ lymphocytopenia: natural history and prognostic factors.
Attack on the clones? Human FOXP3 detection by PCH101, 236A/E7, 206D, and 259D reveals 259D as the outlier with lower sensitivity.
CTLA4 blockade expands FoxP3+ regulatory and activated effector CD4+ T cells in a dose-dependent fashion.
Acquisition of suppressive function by activated human CD4+ CD25- T cells is associated with the expression of CTLA-4 not FoxP3.
Suppression of SIV-specific CD4+ T cells by infant but not adult macaque regulatory T cells: implications for SIV disease progression.
Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype.
Intratumoral balance of regulatory and cytotoxic T cells is associated with prognosis of hepatocellular carcinoma after resection.
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells.
Nigam P, Velu V, Kannanganat S, Chennareddi L, Kwa S, Siddiqui M, Amara RR
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):1690-701
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):1690-701
Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function.
Kmieciak M, Gowda M, Graham L, Godder K, Bear HD, Marincola FM, Manjili MH
Journal of translational medicine 2009 Oct 22;7:89
Journal of translational medicine 2009 Oct 22;7:89
Validated protocol for FoxP3 reveals increased expression in type 1 diabetes patients.
Grant J, Bourcier K, Wallace S, Pan D, Conway A, Seyfert-Margolis V, Wallace PK
Cytometry. Part B, Clinical cytometry 2009 Mar;76(2):69-78
Cytometry. Part B, Clinical cytometry 2009 Mar;76(2):69-78
Evidence of systemic Th2-driven chronic inflammation in patients with metastatic melanoma.
Nevala WK, Vachon CM, Leontovich AA, Scott CG, Thompson MA, Markovic SN, Melanoma Study Group of the Mayo Clinic Cancer Center
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Mar 15;15(6):1931-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Mar 15;15(6):1931-9
FOXP3 expression in blood, synovial fluid and synovial tissue during inflammatory arthritis and intra-articular corticosteroid treatment.
Raghavan S, Cao D, Widhe M, Roth K, Herrath J, Engström M, Roncador G, Banham AH, Trollmo C, Catrina AI, Malmström V
Annals of the rheumatic diseases 2009 Dec;68(12):1908-15
Annals of the rheumatic diseases 2009 Dec;68(12):1908-15
Idiopathic CD4+ lymphocytopenia: natural history and prognostic factors.
Zonios DI, Falloon J, Bennett JE, Shaw PA, Chaitt D, Baseler MW, Adelsberger JW, Metcalf JA, Polis MA, Kovacs SJ, Kovacs JA, Davey RT, Lane HC, Masur H, Sereti I
Blood 2008 Jul 15;112(2):287-94
Blood 2008 Jul 15;112(2):287-94
Attack on the clones? Human FOXP3 detection by PCH101, 236A/E7, 206D, and 259D reveals 259D as the outlier with lower sensitivity.
Pillai V, Karandikar NJ
Blood 2008 Jan 1;111(1):463-4; author reply 464-6
Blood 2008 Jan 1;111(1):463-4; author reply 464-6
CTLA4 blockade expands FoxP3+ regulatory and activated effector CD4+ T cells in a dose-dependent fashion.
Kavanagh B, O'Brien S, Lee D, Hou Y, Weinberg V, Rini B, Allison JP, Small EJ, Fong L
Blood 2008 Aug 15;112(4):1175-83
Blood 2008 Aug 15;112(4):1175-83
Acquisition of suppressive function by activated human CD4+ CD25- T cells is associated with the expression of CTLA-4 not FoxP3.
Zheng Y, Manzotti CN, Burke F, Dussably L, Qureshi O, Walker LS, Sansom DM
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):1683-91
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):1683-91
Suppression of SIV-specific CD4+ T cells by infant but not adult macaque regulatory T cells: implications for SIV disease progression.
Hartigan-O'Connor DJ, Abel K, McCune JM
The Journal of experimental medicine 2007 Oct 29;204(11):2679-92
The Journal of experimental medicine 2007 Oct 29;204(11):2679-92
Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype.
Tran DQ, Ramsey H, Shevach EM
Blood 2007 Oct 15;110(8):2983-90
Blood 2007 Oct 15;110(8):2983-90
Intratumoral balance of regulatory and cytotoxic T cells is associated with prognosis of hepatocellular carcinoma after resection.
Gao Q, Qiu SJ, Fan J, Zhou J, Wang XY, Xiao YS, Xu Y, Li YW, Tang ZY
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2007 Jun 20;25(18):2586-93
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2007 Jun 20;25(18):2586-93
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells.
Liu W, Putnam AL, Xu-Yu Z, Szot GL, Lee MR, Zhu S, Gottlieb PA, Kapranov P, Gingeras TR, Fazekas de St Groth B, Clayberger C, Soper DM, Ziegler SF, Bluestone JA
The Journal of experimental medicine 2006 Jul 10;203(7):1701-11
The Journal of experimental medicine 2006 Jul 10;203(7):1701-11
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Cell extract from HEK293T cells transfected with human FoxP3 cDNA was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-FoxP3 antibody (clone 206D). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated