623801

antibody from BioLegend

Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

623801

Provider

BioLegend

Proper citation

BioLegend Cat#623801, RRID:AB_315971

Product name

Purified anti-FOXP3

Antibody type

Polyclonal

Antigen

Peptide-KLH

Description

The forkhead/winged helix transcription factor FOXP3 is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. The Poly6238 antibody recognizes both human and mouse FOXP3 and has been shown to be useful for Western blotting.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

50 µl

Storage

Upon receipt, store undiluted at -20° C.

References

Submitted references

Intraepithelial CD8+ tumor-infiltrating lymphocytes and a high CD8+/regulatory T cell ratio are associated with favorable prognosis in ovarian cancer.

Sato E, Olson SH, Ahn J, Bundy B, Nishikawa H, Qian F, Jungbluth AA, Frosina D, Gnjatic S, Ambrosone C, Kepner J, Odunsi T, Ritter G, Lele S, Chen YT, Ohtani H, Old LJ, Odunsi K
Proceedings of the National Academy of Sciences of the United States of America 2005 Dec 20;102(51):18538-43

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot analysis of extracts from 293T(lane 1) and 293T transfected with mFOXP3(lane 2) using anti-FOXP3 antibody(poly6238).

Conjugate

Unconjugated