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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX107737 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107737, RRID:AB_1240836
- Product name
- FOXP3 antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX107737 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FOXP3 antibody [C3], C-term detects FOXP3 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FOXP3 protein stained by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FOXP3 antibody [C3], C-term immunoprecipitates FOXP3 protein in IP experiments.IP samples: Jurkat whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of FOXP3 protein by 4 £gg FOXP3 antibody [C3], C-term (GTX107737)10 % SDS-PAGEThe immunoprecipitated FOXP3 protein was detected by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FOXP3 antibody [C3], C-term detects FOXP3 protein at cytoplasm and nucleus in mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
