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LearnMA5-16222
antibody from Invitrogen Antibodies
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [7]
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- Product number
- MA5-16222 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXP3 Monoclonal Antibody (3G3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: antigen standard for FOXP3 (transient overexpression lysate), human Lymph Node protein, mouse lymph node cells.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXP3 in 1) full-length transfected and 2) mock transfected 293 cell lysate using a FOXP3/Scurfin monoclonal antibody (Product # MA5-16222) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXP3 in 1) full-length transfected and 2) mock transfected 293 cell lysate. Samples were incubated in FOXP3 monoclonal antibody (Product # MA5-16222) using a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric detection of FOXP3 in approximately 2x10^6 mouselymph node cells using 100 ng (in 50 µL total volume) of FOXP3/Scurfin monoclonal antibody (Product # MA5-16222).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours. Samples were incubated in FOXP3 monoclonal antibody (Product # MA5-16222) using a dilution of 5 µg/10^6 cells and isotype control (left). Analysis using the FITC conjugate version of antibody. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right). Samples were incubated in FOXP3 monoclonal antibody (Product # MA5-16222) using a dilution of 0.1 µg/10^6 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours. Samples were incubated in FOXP3 monoclonal antibody (Product # MA5-16222) using a dilution of 1 µg/10^6 cells and isotype control (left). Analysis using the azide free version of antibody. Products used: anti-CD4 FITC conjugate, anti-CD25 APC conjugate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right). Samples were incubated in FOXP3 monoclonal antibody (Product # MA5-16222) using a dilution of 0.5 µg/10^6 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours. Samples were incubated in FOXP3 monoclonal antibody (Product # MA5-16222) using a dilution of 1 µg/10^6 cells and isotype control (left). Antibody conjugated to Allophycocyanin. Products used: anti-CD4 FITC conjugate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FOXP3 in 2 x 10^6 mouse lymph node cells. Samples were incubated in FOXP3 monoclonal antibody (Product # MA5-16222) using a dilution of 0.1 µg. Analysis using the azide free and PE conjugated antibody.
