MA5-16226

antibody from Invitrogen Antibodies

Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-16226

Provider

Invitrogen Antibodies

Product name

FOXP3 Monoclonal Antibody (16J4G6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

Isotype

IgM

Antibody clone number

16J4G6

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FOXP3D and full-length (wild-type) transfected 293 cell lysate using a FOXP3D2 (exon 2 deleted) monoclonal antibody (Product # MA5-16226)and a FOXP3/Scurfin monoclonal antibody (Product # MA5-16222). At 1 µg/mL, a FOXP3D2 (exon 2 deleted) monoclonal antibody (Product # MA5-16226) specifically detects the FOXP3 (Lane 1); it does not detect full-length FOXP3 (Lane 2).In contrast, a FOXP3/Scurfin monoclonal antibody (Product # MA5-16222) detects both FOXP3D (Lane 3) and full-length (Lane 4) FOXP3.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FOXP3 in FOXP3D2 and full-length (wild-type) transfected 293 cell lysate. Samples were incubated in FOXP3 monoclonal antibody (Product # MA5-16226 using a dilution of 1 µg/mL. Specifically detects the FOXP3D2 (Lane 1); it does not detect full-length FOXP3 (Lane 2). In contrast, FOXP3/Scurfin detects both FOXP3D2 (Lane 3) and full-length (Lane 4) FOXP3.