PA5-23169
antibody from Invitrogen Antibodies
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-23169 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Boosting Akt Pathway by Rupatadine Modulates Th17/Tregs Balance for Attenuation of Isoproterenol-Induced Heart Failure in Rats.
Ahmed LA, Mohamed AF, Abd El-Haleim EA, El-Tanbouly DM
Frontiers in pharmacology 2021;12:651150
Frontiers in pharmacology 2021;12:651150
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXP3 in (A) FOXP3 transfected and (B) mock transfected cell lysate. Samples were incubated in FOXP3 polyclonal antibody (Product # PA5-23169 using a dilution of 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FOXP3 in formalin fixed paraffin-embedded (FFPE) human tonsil. Samples were incubated in FOXP3 polyclonal antibody (Product # PA5-23169) using a dilution of 1:50. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 Effect of RUP with or without wortmannin on ISO-induced changes in protein expression of (A) Foxp3 and (B) RORgammat in addition to (C) Foxp3/RORgammat ratio and myocardial content of (D) IL-17. Each value represents the mean of six experiments and error bars represent SD. Statistical analysis was done using One way ANOVA followed by Tukey's post-hoc test where a p < 0.05 vs. normal, b p < 0.05 vs. ISO, c p < 0.05 vs. RUP.