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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
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- Product number
- IC3240A - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat FoxP3 APC-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human FoxP3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxD3 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Red dye
- Antigen sequence
Q9BZS1- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references A small-molecule inhibitor of macrophage migration inhibitory factor for the treatment of inflammatory disease.
Kithcart AP, Cox GM, Sielecki T, Short A, Pruitt J, Papenfuss T, Shawler T, Gienapp I, Satoskar AR, Whitacre CC
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Nov;24(11):4459-66
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Nov;24(11):4459-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Mouse Anti-Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C) or (B) Mouse Anti-Human IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB1020P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat Anti-Mouse IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB2438P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).
