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LearnNB600-243
antibody from Novus Biologicals
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
ELISA
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NB600-243 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-243, RRID:AB_2210244
- Product name
- Goat Polyclonal FoxP3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse.
Brunkow ME, Jeffery EW, Hjerrild KA, Paeper B, Clark LB, Yasayko SA, Wilkinson JE, Galas D, Ziegler SF, Ramsdell F
Nature genetics 2001 Jan;27(1):68-73
Nature genetics 2001 Jan;27(1):68-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - Staining left panel of paraffin embedded Mouse Thymus.Right panel shows staining without primary antibody as negative control. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - Paraffin embedded Mouse Spleen shows patches of stained cells in the Germinal Centre. Recommended antibody concentration, 2 - 4 ug/mL.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: FoxP3 Antibody [NB600-243] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
