UM800099

antibody from Invitrogen Antibodies

Targeting: PYM1 PYM, WIBG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

UM800099

Provider

Invitrogen Antibodies

Product name

WIBG Monoclonal Antibody (UMAB207), UltraMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

UMAB207

Vial size

100 µL

Concentration

0.5-1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (15 µg) from 9 Human tissue by using anti-WIBG monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500.) Dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of cell lysates (35 µg) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-WIBG mouse monoclonal antibody (UM800099, green, 1:100). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded normal/normal adjacent breast with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic and some nuclear in normal/normal adjacent breast tissue.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded breast carcinoma with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded thyroid with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded pancreas with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded kidney with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded ovarian cancer with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded colon cancer with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-WIBG mouse monoclonal antibody (UM800099). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)