PAB28110

antibody from Abnova Corporation

Targeting: CCDC97 FLJ40267, MGC20255

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28110

Provider

Abnova Corporation

Product name

CCDC97 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CCDC97.

Antigen sequence

PDKGCIEPGPGHWGELSRTPVPSKPQDKVEAAEAT
PVALDSDTSGAENAAVSAMLHAVAASRLPVCSQQQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with CCDC97 polyclonal antibody (Cat # PAB28110) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with CCDC97 polyclonal antibody (Cat # PAB28110) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with CCDC97 polyclonal antibody (Cat # PAB28110) shows strong nuclear positivity in cells in seminiferus ducts and nuclear membrane staining in Leydig cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)