25671-1-AP

antibody from Proteintech Group

Targeting: CHCHD10 C22orf16, MIX17A, N27C7-4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25671-1-AP

Provider

Proteintech Group

Product name

CHCHD10 antibody

Antibody type

Polyclonal

Description

KD/KO validated CHCHD10 antibody (Cat. #25671-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Pregnancy precipitates metabolic imbalance and accelerates death in an animal model of mitochondrial cardiomyopathy.

Sayles NM, Casalena G, Zhao D, Dellinger RW, Holmes HE, Manzo O, Galkin A, Di Lorenzo A, Manfredi G
Molecular metabolism 2026 May;107:102352

---

Age-Associated Dysregulation of Postsynaptic Mitochondria Perturbs Reinnervation Kinetics.

Guzman SD, Fraczek PM, Itsani K, Furati EK, Juros D, Kenney G, Valdez G, Chakkalakal JV, Aguilar CA
Aging cell 2026 Jan;25(1):e70355

---

LncRNA LINC00667 inhibits breast cancer progression by regulating POTEE to suppress mitochondrial oxidative phosphorylation.

Yu Z, Xu K, Shang Y, Geng C, Zhang Y, Wang L
Cellular signalling 2025 Nov;135:112074

---

CHCHD2 mutant mice link mitochondrial deficits to PD pathophysiology.

Liao SC, Kano K, Phanse S, Nguyen M, Margolis E, Fu Y, X Meng J, Moutaoufik MT, Chatterton Z, Saccon T, Broderick K, Aoki H, Simms J, Suteja FX, Sei Y, Huang EJ, McAvoy K, Manfredi G, Halliday G, Babu M, Nakamura K
Science advances 2025 Nov 14;11(46):eadu0726

---

Longitudinal single-cell RNA sequencing reveals a heterogeneous response of plasma cells to colonic inflammation.

Wen X, Li W, Li S, Chen D, Zheng J, Wang X, Zhang C, Liu Y, Ning Y, Jia R, Li P, Ji M, Ji C, Li J, Guo W
International journal of biological macromolecules 2025 Mar;294:139307

---

Expression profile of Chchd10 gene during testicular development.

Jiang X, Sun S, Han F, Sun L, Gu J, Cao J, Liu J
Gene expression patterns : GEP 2025 Jun;55:119395

---

Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson's disease models.

Gao G, Shi Y, Deng HX, Krainc D
Nature communications 2025 Feb 26;16(1):1982

---

Lineage plasticity of the integrated stress response is a hallmark of cancer evolution.

Diao S, Zou JY, Wang S, Ghaddar N, Chan JE, Kim H, Poulain N, Koumenis C, Hatzoglou M, Walter P, Sonenberg N, Le Quesne J, Tammela T, Koromilas AE
bioRxiv : the preprint server for biology 2025 Feb 13;

---

Amyloid fibril structures link CHCHD10 and CHCHD2 to neurodegeneration.

Lv G, Sayles NM, Huang Y, Mancinelli C, McAvoy K, Shneider NA, Manfredi G, Kawamata H, Eliezer D
Nature communications 2025 Aug 2;16(1):7121

---

Clioquinol induces mitochondrial toxicity in SH-SY5Y neuroblastoma cells by affecting the respiratory chain complex IV and OPA1 dynamin-like GTPase.

Katsuyama M, Arakawa N, Yaoi T, Kimura E, Matsumoto M, Iwata K, Umemura A, Yabe-Nishimura C
FEBS letters 2025 Apr;599(8):1135-1145

---

Chchd10: A Novel Metabolic Sensor Modulating Adipose Tissue Homeostasis.

Wu X, Zhang Z, Li J, Zong J, Yuan L, Shu L, Cheong LY, Huang X, Jiang M, Ping Z, Xu A, Hoo RLC
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Apr;12(15):e2408763

---

Loss of CHCHD2 Stability Coordinates with C1QBP/CHCHD2/CHCHD10 Complex Impairment to Mediate PD-Linked Mitochondrial Dysfunction.

Ren YL, Jiang Z, Wang JY, He Q, Li SX, Gu XJ, Qi YR, Zhang M, Yang WJ, Cao B, Li JY, Wang Y, Chen YP
Molecular neurobiology 2024 Oct;61(10):7968-7988

---

Mitochondrial protein CHCHD10 inhibits NDV replication and reduces pathological changes.

Yu X, Jiang H, Li J, Ding J, Wu T, Chen K, Ding Z, Xu X
Veterinary microbiology 2024 Mar;290:109986

---

High fat diet ameliorates mitochondrial cardiomyopathy in CHCHD10 mutant mice.

Southwell N, Manzo O, Bacman S, Zhao D, Sayles NM, Dash J, Fujita K, D'Aurelio M, Di Lorenzo A, Manfredi G, Kawamata H
EMBO molecular medicine 2024 Jun;16(6):1352-1378

---

CHCHD2 mutant mice display mitochondrial protein accumulation and disrupted energy metabolism.

Liao SC, Kano K, Phanse S, Nguyen M, Margolis E, Fu Y, Meng J, Moutaoufik MT, Chatterton Z, Aoki H, Simms J, Hsieh I, Suteja F, Sei Y, Huang EJ, McAvoy K, Manfredi G, Halliday G, Babu M, Nakamura K
bioRxiv : the preprint server for biology 2024 Aug 31;

---

The identification of high-performing antibodies for Coiled-coil-helix-coiled-coil-helix domain containing protein 10 (CHCHD10) for use in Western Blot, immunoprecipitation and immunofluorescence.

Ayoubi R, Alshafie W, Southern K, McPherson PS, Laflamme C, NeuroSGC/YCharOS/EDDU collaborative group
F1000Research 2023;12:403

---

CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis.

Ding M, Ma YJ, Du RQ, Zhou WY, Dou X, Yang QQ, Tang Y, Qian SW, Liu Y, Pan DN, Tang QQ, Liu Y
Diabetes 2022 Sep 1;71(9):1862-1879

---

Mouse midbrain dopaminergic neurons survive loss of the PD-associated mitochondrial protein CHCHD2.

Nguyen MK, McAvoy K, Liao SC, Doric Z, Lo I, Li H, Manfredi G, Nakamura K
Human molecular genetics 2022 May 4;31(9):1500-1518

---

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Sayles NM, Southwell N, McAvoy K, Kim K, Pesini A, Anderson CJ, Quinzii C, Cloonan S, Kawamata H, Manfredi G
Cell reports 2022 Mar 8;38(10):110475

---

Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.

Stein CS, McLendon JM, Witmer NH, Boudreau RL
Molecular therapy. Nucleic acids 2022 Jun 14;28:1-15

---

CHCHD2 and CHCHD10 regulate mitochondrial dynamics and integrated stress response.

Ruan Y, Hu J, Che Y, Liu Y, Luo Z, Cheng J, Han Q, He H, Zhou Q
Cell death & disease 2022 Feb 16;13(2):156

---

Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing.

Li H, Peng H, Hong W, Wei Y, Tian H, Huang X, Jia L, Zheng J, Duan T, He Q, Wang K
Cells 2022 Dec 25;12(1)

---

TDP-43 and PINK1 mediate CHCHD10(S59L) mutation-induced defects in Drosophila and in vitro.

Baek M, Choe YJ, Bannwarth S, Kim J, Maitra S, Dorn GW 2nd, Taylor JP, Paquis-Flucklinger V, Kim NC
Nature communications 2021 Mar 26;12(1):1924

---

Loss of mitochondrial protein CHCHD10 in skeletal muscle causes neuromuscular junction impairment.

Xiao Y, Zhang J, Shu X, Bai L, Xu W, Wang A, Chen A, Tu WY, Wang J, Zhang K, Luo B, Shen C
Human molecular genetics 2020 Jul 21;29(11):1784-1796

---

CHCHD10 is involved in the development of Parkinson's disease caused by CHCHD2 loss-of-function mutation p.T61I.

Mao C, Wang H, Luo H, Zhang S, Xu H, Zhang S, Rosenblum J, Wang Z, Zhang Q, Tang M, Shepard MJ, Wang X, Wang Y, Zhuang Z, Shi C, Xu Y
Neurobiology of aging 2019 Mar;75:38-41

---

Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.

Peng J, Sheng AL, Xiao Q, Shen L, Ju XC, Zhang M, He ST, Wu C, Luo ZG
Journal of molecular cell biology 2019 Aug 19;11(8):636-648

---

PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction.

Zhou W, Ma D, Sun AX, Tran HD, Ma DL, Singh BK, Zhou J, Zhang J, Wang D, Zhao Y, Yen PM, Goh E, Tan EK
Human molecular genetics 2019 Apr 1;28(7):1100-1116

---

Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.

Straub IR, Janer A, Weraarpachai W, Zinman L, Robertson J, Rogaeva E, Shoubridge EA
Human molecular genetics 2018 Jan 1;27(1):178-189

---

CHCHD10 mutations p.R15L and p.G66V cause motoneuron disease by haploinsufficiency.

Brockmann SJ, Freischmidt A, Oeckl P, Müller K, Ponna SK, Helferich AM, Paone C, Reinders J, Kojer K, Orth M, Jokela M, Auranen M, Udd B, Hermann A, Danzer KM, Lichtner P, Walther P, Ludolph AC, Andersen PM, Otto M, Kursula P, Just S, Weishaupt JH
Human molecular genetics 2018 Feb 15;27(4):706-715

---

Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity.

Woo JA, Liu T, Trotter C, Fang CC, De Narvaez E, LePochat P, Maslar D, Bukhari A, Zhao X, Deonarine A, Westerheide SD, Kang DE
Nature communications 2017 Jun 6;8:15558

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse heart tissue were subjected to SDS PAGE followed by western blot with 25671-1-AP( CHCHD10 Antibody) at dilution of 1:600

Other comments

WB

Appendix

25671-1-AP.pdf

Show more

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of A431 cells using 25671-1-AP( CHCHD10 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Other comments

IF

Appendix

25671-1-AP.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 25671-1-AP( CHCHD10 Antibody) at dilution of 1:50 (under 10x lens)

Other comments

IHC

Appendix

25671-1-AP.pdf

Show more