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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-22282 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal RGNEF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RGNEF Antibody [NBP2-22282] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22282 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RGNEF Antibody [NBP2-22282] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RGNEF antibody NBP2-22282 used for IP at 6 ug/mg lysate. RGNEF was also immunoprecipitated by rabbit anti-RGNEF antibody. For blotting immunoprecipitated RGNEF, NBP2-22282 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
