LS-B15874

antibody from LSBio

Targeting: ARHGEF28 p190RhoGEF, RGNEF, RIP2

Western blot (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B15874

Provider

LSBio

Product name

IHC-plus™ ARHGEF28 Antibody (aa1655-1705) LS-B15874

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human p190RhoGEF by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-p190RhoGEF antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human p190RhoGEF by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-p190RhoGEF antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human and mouse Staufen1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-Staufen1 antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.