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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- A303-930A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p190RhoGEF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human p190RhoGEF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p190RhoGEF antibody A303-930A (lot A303-930A-1) used for IP at 6 µg/mg lysate. p190RhoGEF was also immunoprecipitated by rabbit anti-p190RhoGEF antibody BL14155. For blotting immunoprecipitated p190RhoGEF, A303-930A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human p190RhoGEF by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), 293T (50µg), and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-p190RhoGEF antibody A303-930A (lot A303-930A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
