PA5-24997

antibody from Invitrogen Antibodies

Targeting: SEMA3C SEMAE, SemE

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24997

Provider

Invitrogen Antibodies

Product name

SEMA3C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model.

Valiulytė I, Curkūnavičiūtė R, Ribokaitė L, Kazlauskas A, Vaitkevičiūtė M, Skauminas K, Valančiūtė A
International journal of molecular sciences 2019 Nov 12;20(22)

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Importance of the putative furin recognition site 742RNRR745 for antiangiogenic Sema3C activity in vitro.

Valiulyte I, Preitakaite V, Tamasauskas A, Kazlauskas A
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2018 Oct 8;51(11):e7786

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Verification of the constructed U87t-Sema3C cell line. ( A ) Microscopic images. Pictures were taken after 2 days of incubation with tetracycline. Fluorescence indicates overexpression of Sema3C protein. -Tet--no tetracycline, +Tet--1 ug/mL of tetracycline was added to the cell media. Scale bar, 100 mum. ( B ) Western blot analysis of Sema3C protein expression in U87t-Sema3C cells and cell medium. Black bar indicates Sema3C protein, approximately 85 kDa in size. Lanes 1 and 4--protein marker; lanes 2 and 5--untreated cells; lanes 3 and 6--cells treated with 1 ug/mL of tetracycline.