13497-1-AP

antibody from Proteintech Group

Targeting: OTX2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

13497-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#13497-1-AP, RRID:AB_2157176

Product name

OTX2 antibody

Antibody type

Polyclonal

Description

KD/KO validated OTX2 antibody (Cat. #13497-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Cell stem cell 2026 Jan 8;33(1):91-107.e9

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Generation of spatially patterned human neural tube-like structures using microfluidic gradient devices.

Xue X, Rahman OM, Sun S, Bok J, Tang A, Fu J
Nature protocols 2026 Apr;21(4):1730-1753

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A tumor-associated photoreceptor signature unifies distinct central nervous system malignancies.

Gudenas BL, Ahmad ST, Englinger B, Liu APY, Zhao M, Paul L, Hadley J, Li Y, Batts M, Mittal P, Wu SC, Lewis SA, Han K, Soliman T, Lin H, Janke L, Meredith D, Pfaff E, Gojo J, Cotter J, Klimo P Jr, Boop FA, Gajjar A, Robinson GW, Rosén G, Alexandrescu S, Jones DTW, Orr BA, Swartling FJ, Filbin MG, Northcott PA
Cancer cell 2026 Apr 13;44(4):831-845.e10

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SOX2 commands LIM homeobox transcription factors in choroid plexus development and tumorigenesis.

Faltings LJ, Lin F, Zahran M, Jalili H, Siluveru A, Chaudry M, Wachsmuth LM, Guru N, Schoof M, Cao P, Huang Y, Mian N, Moon S, Zahran A, Green K, Modi S, Umer M, Virga JQ, Zhao YT, Schüller U, Li Q, Zhao H
Neuro-oncology 2025 Sep 17;27(8):2006-2022

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OTX2 expression contributes progression of gastric cancer in young adults.

Zhai J, Lyu T, Guo Y, An Y, Xiang Y, Xie L, Zeng C
Scientific reports 2025 May 9;15(1):16146

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Activating Wnt1/β-Catenin signaling pathway to restore Otx2 expression in the dopaminergic neurons of ventral midbrain.

Li Z, Duan J, Cao A, Gong Z, Liu H, Shen D, Ye T, Zhu S, Cen Q, He S, He Y, Zheng C, Lin X
Experimental neurology 2025 Jun;388:115216

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In-depth inference of transcriptional regulatory networks reveals NPM1 as a therapeutic ribosomal regulator in MYC-amplified medulloblastoma.

Chen T, Chen H, Xia M, Liao Y, Li H, Dong X, Lin Y, Zhou W
NPJ precision oncology 2025 Jan 10;9(1):10

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Neonatal hypoxia-ischemia alters the events governing the hippocampal critical period of postnatal synaptic plasticity leading to deficits in working memory in mice.

Parmar P, Spahic H, Lechner C, St Pierre M, Carlin K, Nugent M, Chavez-Valdez R
Neurobiology of disease 2024 Nov;202:106722

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A stepwise mode of TGFβ-SMAD signaling and DNA methylation regulates naïve-to-primed pluripotency and differentiation.

Zhao B, Yu X, Shi J, Ma S, Li S, Shi H, Xia S, Ye Y, Zhang Y, Du Y, Wang Q
Nature communications 2024 Nov 22;15(1):10123

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ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells.

Lu D, Zhi Y, Su H, Lin X, Lin J, Shi Y, Yi W, Hong C, Zhang T, Fu Z, Chen LY, Zhao Z, Li R, Xu Z, Chen W, Wang N, Xu D
Cell reports 2024 Mar 26;43(3):113818

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Homology and the evolution of vocal folds in the novel avian voice box.

Longtine C, Eliason CM, Mishkind D, Lee C, Chiappone M, Goller F, Love J, Kingsley EP, Clarke JA, Tabin CJ
Current biology : CB 2024 Feb 5;34(3):461-472.e7

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Restoring vision in adult amblyopia by enhancing plasticity through deletion of the transcriptional repressor REST.

Shmal D, Mantero G, Floss T, Benfenati F, Maya-Vetencourt JF
iScience 2024 Apr 19;27(4):109507

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MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration.

Du J, Liao W, Wang H, Hou G, Liao M, Xu L, Huang J, Yuan K, Chen X, Zeng Y
Signal transduction and targeted therapy 2023 Sep 15;8(1):351

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O-GlcNAcylation regulates OTX2's proteostasis.

Wulff-Fuentes E, Boakye J, Kroenke K, Berendt RR, Martinez-Morant C, Pereckas M, Hanover JA, Olivier-Van Stichelen S
iScience 2023 Nov 17;26(11):108184

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AAV-mediated gene augmentation therapy of CRB1 patient-derived retinal organoids restores the histological and transcriptional retinal phenotype.

Boon N, Lu X, Andriessen CA, Moustakas I, Buck TM, Freund C, Arendzen CH, Böhringer S, Mei H, Wijnholds J
Stem cell reports 2023 May 9;18(5):1123-1137

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Characterization and AAV-mediated CRB gene augmentation in human-derived CRB1(KO) and CRB1(KO)CRB2(+/-) retinal organoids.

Boon N, Lu X, Andriessen CA, Orlovà M, Quinn PMJ, Boon CJF, Wijnholds J
Molecular therapy. Methods & clinical development 2023 Dec 14;31:101128

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Unified rhombic lip origins of group 3 and group 4 medulloblastoma.

Smith KS, Bihannic L, Gudenas BL, Haldipur P, Tao R, Gao Q, Li Y, Aldinger KA, Iskusnykh IY, Chizhikov VV, Scoggins M, Zhang S, Edwards A, Deng M, Glass IA, Overman LM, Millman J, Sjoboen AH, Hadley J, Golser J, Mankad K, Sheppard H, Onar-Thomas A, Gajjar A, Robinson GW, Hovestadt V, Orr BA, Patay Z, Millen KJ, Northcott PA
Nature 2022 Sep;609(7929):1012-1020

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Elimination of senescent cells inhibits epithelial-mesenchymal transition of retinal pigment epithelial cells.

Gao F, Wang L, Wu B, Ou Q, Tian H, Xu J, Jin C, Zhang J, Wang J, Lu L, Xu GT
Experimental eye research 2022 Oct;223:109207

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Dissecting super-enhancer driven transcriptional dependencies reveals novel therapeutic strategies and targets for group 3 subtype medulloblastoma.

Li M, Han Y, Wang C, Kang W, Jiang W, Zhang L, Tang Y
Journal of experimental & clinical cancer research : CR 2022 Oct 22;41(1):311

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Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages.

Sun Y, Wang L, Zhu T, Wu B, Wang G, Luo Z, Li C, Wei W, Liu Z
Cell reports 2022 Mar 22;38(12):110542

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Exogenous Otx2 protects midbrain dopaminergic neurons from MPP(+) by interacting with ATP5a1 and promoting ATP synthesis.

Xue J, Li Y, Qi Y, Zhang Z, Tang X
Neurotoxicology 2022 Jul;91:211-217

---

Binge-Like Ethanol Drinking Increases Otx2, Wnt1, and Mdk Gene Expression in the Ventral Tegmental Area of Adult Mice.

Coles C, Lasek AW
Neuroscience insights 2021;16:26331055211009850

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Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins.

Pereira Luppi M, Azcorra M, Caronia-Brown G, Poulin JF, Gaertner Z, Gatica S, Moreno-Ramos OA, Nouri N, Dubois M, Ma YC, Ramakrishnan C, Fenno L, Kim YS, Deisseroth K, Cicchetti F, Dombeck DA, Awatramani R
Cell reports 2021 Nov 9;37(6):109975

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A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation.

Wang J, Rattner A, Nathans J
eLife 2021 Nov 16;10

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OTX2 regulates CFTR expression during endoderm differentiation and occupies 3' cis-regulatory elements.

Kerschner JL, Paranjapye A, NandyMazumdar M, Yin S, Leir SH, Harris A
Developmental dynamics : an official publication of the American Association of Anatomists 2021 May;250(5):684-700

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Cis-regulatory dissection of cone development reveals a broad role for Otx2 and Oc transcription factors.

Lonfat N, Wang S, Lee C, Garcia M, Choi J, Park PJ, Cepko C
Development (Cambridge, England) 2021 May 1;148(9)

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Nuclear NAD(+)-biosynthetic enzyme NMNAT1 facilitates development and early survival of retinal neurons.

Sokolov D, Sechrest ER, Wang Y, Nevin C, Du J, Kolandaivelu S
eLife 2021 Dec 8;10

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Effective Inhibition of MYC-Amplified Group 3 Medulloblastoma Through Targeting EIF4A1.

Zhao Y, Li T, Tian S, Meng W, Sui Y, Yang J, Wang B, Liang Z, Zhao H, Han Y, Tang Y, Zhang L, Ma J
Cancer management and research 2020;12:12473-12485

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Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Qian H, Kang X, Hu J, Zhang D, Liang Z, Meng F, Zhang X, Xue Y, Maimon R, Dowdy SF, Devaraj NK, Zhou Z, Mobley WC, Cleveland DW, Fu XD
Nature 2020 Jun;582(7813):550-556

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ZIC3 Controls the Transition from Naive to Primed Pluripotency.

Yang SH, Andrabi M, Biss R, Murtuza Baker S, Iqbal M, Sharrocks AD
Cell reports 2019 Jun 11;27(11):3215-3227.e6

---

DMBX1 promotes tumor proliferation and regulates cell cycle progression via repressing OTX2-mediated transcription of p21 in lung adenocarcinoma cell.

Luo J, Liu K, Yao Y, Sun Q, Zheng X, Zhu B, Zhang Q, Xu L, Shen Y, Ren B
Cancer letters 2019 Jul 1;453:45-56

---

Molecular network of miR-1343 regulates the pluripotency of porcine pluripotent stem cells via repressing OTX2 expression.

Xie Y, Cao H, Zhang Z, Zhang S, Wang H
RNA biology 2019 Jan;16(1):82-92

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Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons Recover Locomotive Defects of Nonhuman Primate Models of Parkinson's Disease.

Wang YK, Zhu WW, Wu MH, Wu YH, Liu ZX, Liang LM, Sheng C, Hao J, Wang L, Li W, Zhou Q, Hu BY
Stem cell reports 2018 Jul 10;11(1):171-182

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Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.

Li Y, Wang L, Zhang X, Huang M, Li S, Wang X, Chen L, Jiang B, Yang Y
Neuropharmacology 2018 Jan;128:207-220

---

Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.

Peña CJ, Kronman HG, Walker DM, Cates HM, Bagot RC, Purushothaman I, Issler O, Loh YE, Leong T, Kiraly DD, Goodman E, Neve RL, Shen L, Nestler EJ
Science (New York, N.Y.) 2017 Jun 16;356(6343):1185-1188

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Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.

Johann PD, Erkek S, Zapatka M, Kerl K, Buchhalter I, Hovestadt V, Jones DTW, Sturm D, Hermann C, Segura Wang M, Korshunov A, Rhyzova M, Gröbner S, Brabetz S, Chavez L, Bens S, Gröschel S, Kratochwil F, Wittmann A, Sieber L, Geörg C, Wolf S, Beck K, Oyen F, Capper D, van Sluis P, Volckmann R, Koster J, Versteeg R, von Deimling A, Milde T, Witt O, Kulozik AE, Ebinger M, Shalaby T, Grotzer M, Sumerauer D, Zamecnik J, Mora J, Jabado N, Taylor MD, Huang A, Aronica E, Bertoni A, Radlwimmer B, Pietsch T, Schüller U, Schneppenheim R, Northcott PA, Korbel JO, Siebert R, Frühwald MC, Lichter P, Eils R, Gajjar A, Hasselblatt M, Pfister SM, Kool M
Cancer cell 2016 Mar 14;29(3):379-393

---

Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency.

Yang SH, Kalkan T, Morissroe C, Marks H, Stunnenberg H, Smith A, Sharrocks AD
Cell reports 2014 Jun 26;7(6):1968-81

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 13497-1-AP(OTX2 antibody) at dilution of 1:500

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 13497-1-AP(OTX2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 13497-1-AP(OTX2 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue