Antibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1979 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Otx2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Otx2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P32243
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene.
Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations.
Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene.
Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene.
Photoreceptor degeneration in microphthalmia (Mitf) mice: partial rescue by pigment epithelium-derived factor.
Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations.
Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation.
Small non-coding RNA landscape of extracellular vesicles from human stem cells.
Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of Müller Glia-Derived Progenitor Cells in the Avian Retina.
Tau Deficiency Down-Regulated Transcription Factor Orthodenticle Homeobox 2 Expression in the Dopaminergic Neurons in Ventral Tegmental Area and Caused No Obvious Motor Deficits in Mice.
In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells.
A new genetically engineered mouse model of choroid plexus carcinoma.
Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143.
Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL.
Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming.
Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells.
Small molecule Photoregulin3 prevents retinal degeneration in the Rho(P23H) mouse model of retinitis pigmentosa.
Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-α.
Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice.
Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages.
Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours.
Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.
Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
Riera M, Patel A, Corcostegui B, Chang S, Corneo B, Sparrow JR, Pomares E
Stem cell research 2019 Mar;35:101386
Stem cell research 2019 Mar;35:101386
Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations.
Riera M, Patel A, Corcostegui B, Chang S, Sparrow JR, Pomares E, Corneo B
Stem cell research 2019 Mar;35:101385
Stem cell research 2019 Mar;35:101385
Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene.
Domingo-Prim J, Riera M, Burés-Jelstrup A, Corcostegui B, Pomares E
Stem cell research 2019 Jul;38:101468
Stem cell research 2019 Jul;38:101468
Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene.
Maguire JA, Gagne AL, Gonzalez-Alegre P, Davidson BL, Shakkottai V, Gadue P, French DL
Stem cell research 2019 Jan;34:101361
Stem cell research 2019 Jan;34:101361
Photoreceptor degeneration in microphthalmia (Mitf) mice: partial rescue by pigment epithelium-derived factor.
Chen Y, Yang J, Geng H, Li L, Li J, Cheng B, Ma X, Li H, Hou L
Disease models & mechanisms 2019 Jan 11;12(1)
Disease models & mechanisms 2019 Jan 11;12(1)
Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations.
Riera M, Patel A, Burés-Jelstrup A, Corcostegui B, Chang S, Pomares E, Corneo B, Sparrow JR
Stem cell research 2019 Apr;36:101389
Stem cell research 2019 Apr;36:101389
Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation.
Worley KE, Chin AS, Wan LQ
Stem cells international 2018;2018:1848605
Stem cells international 2018;2018:1848605
Small non-coding RNA landscape of extracellular vesicles from human stem cells.
Kaur S, Abu-Shahba AG, Paananen RO, Hongisto H, Hiidenmaa H, Skottman H, Seppänen-Kaijansinkko R, Mannerström B
Scientific reports 2018 Oct 19;8(1):15503
Scientific reports 2018 Oct 19;8(1):15503
Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of Müller Glia-Derived Progenitor Cells in the Avian Retina.
Todd L, Suarez L, Quinn C, Fischer AJ
Stem cells (Dayton, Ohio) 2018 Mar;36(3):392-405
Stem cells (Dayton, Ohio) 2018 Mar;36(3):392-405
Tau Deficiency Down-Regulated Transcription Factor Orthodenticle Homeobox 2 Expression in the Dopaminergic Neurons in Ventral Tegmental Area and Caused No Obvious Motor Deficits in Mice.
Tang X, Jiao L, Zheng M, Yan Y, Nie Q, Wu T, Wan X, Zhang G, Li Y, Wu S, Jiang B, Cai H, Xu P, Duan J, Lin X
Neuroscience 2018 Mar 1;373:52-59
Neuroscience 2018 Mar 1;373:52-59
In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells.
Harrison SE, Sozen B, Zernicka-Goetz M
Nature protocols 2018 Jul;13(7):1586-1602
Nature protocols 2018 Jul;13(7):1586-1602
A new genetically engineered mouse model of choroid plexus carcinoma.
El Nagar S, Zindy F, Moens C, Martin L, Plassard D, Roussel MF, Lamonerie T, Billon N
Biochemical and biophysical research communications 2018 Feb 5;496(2):568-574
Biochemical and biophysical research communications 2018 Feb 5;496(2):568-574
Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143.
Baulier E, Garcia Diaz A, Corneo B, Farber DB
Stem cell research 2018 Dec;33:274-277
Stem cell research 2018 Dec;33:274-277
Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL.
Gagne AL, Maguire JA, Gandre-Babbe S, Chou ST, Tasian SK, Loh ML, Weiss MJ, Gadue P, French DL
Stem cell research 2018 Aug;31:157-160
Stem cell research 2018 Aug;31:157-160
Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming.
Senft AD, Costello I, King HW, Mould AW, Bikoff EK, Robertson EJ
Cell reports 2018 Aug 21;24(8):1977-1985.e7
Cell reports 2018 Aug 21;24(8):1977-1985.e7
Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells.
Kim JY, Lee JS, Hwang HS, Lee DR, Park CY, Jung SJ, You YR, Kim DS, Kim DW
Experimental & molecular medicine 2018 Apr 13;50(4):24
Experimental & molecular medicine 2018 Apr 13;50(4):24
Small molecule Photoregulin3 prevents retinal degeneration in the Rho(P23H) mouse model of retinitis pigmentosa.
Nakamura PA, Shimchuk AA, Tang S, Wang Z, DeGolier K, Ding S, Reh TA
eLife 2017 Nov 17;6
eLife 2017 Nov 17;6
Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-α.
Mathis T, Housset M, Eandi C, Beguier F, Touhami S, Reichman S, Augustin S, Gondouin P, Sahel JA, Kodjikian L, Goureau O, Guillonneau X, Sennlaub F
Aging cell 2017 Feb;16(1):173-182
Aging cell 2017 Feb;16(1):173-182
Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
Edri R, Yaffe Y, Ziller MJ, Mutukula N, Volkman R, David E, Jacob-Hirsch J, Malcov H, Levy C, Rechavi G, Gat-Viks I, Meissner A, Elkabetz Y
Nature communications 2015 Mar 23;6:6500
Nature communications 2015 Mar 23;6:6500
Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
Zou M, Luo H, Xiang M
Developmental dynamics : an official publication of the American Association of Anatomists 2015 Jan;244(1):86-97
Developmental dynamics : an official publication of the American Association of Anatomists 2015 Jan;244(1):86-97
SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice.
Wu Q, Fukuda K, Weinstein M, Graff JM, Saga Y
Development (Cambridge, England) 2015 Feb 1;142(3):575-86
Development (Cambridge, England) 2015 Feb 1;142(3):575-86
Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
Di Giovannantonio LG, Di Salvio M, Omodei D, Prakash N, Wurst W, Pierani A, Acampora D, Simeone A
Development (Cambridge, England) 2014 Jan;141(2):377-88
Development (Cambridge, England) 2014 Jan;141(2):377-88
Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages.
Stott SR, Metzakopian E, Lin W, Kaestner KH, Hen R, Ang SL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 May 1;33(18):8022-34
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 May 1;33(18):8022-34
Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours.
Rijlaarsdam MA, van Herk HA, Gillis AJ, Stoop H, Jenster G, Martens J, van Leenders GJ, Dinjens W, Hoogland AM, Timmermans M, Looijenga LH
British journal of cancer 2011 Sep 6;105(6):854-63
British journal of cancer 2011 Sep 6;105(6):854-63
Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.
Zeng H, Guo M, Martins-Taylor K, Wang X, Zhang Z, Park JW, Zhan S, Kronenberg MS, Lichtler A, Liu HX, Chen FP, Yue L, Li XJ, Xu RH
PloS one 2010 Jul 29;5(7):e11853
PloS one 2010 Jul 29;5(7):e11853
Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
Di Salvio M, Di Giovannantonio LG, Acampora D, Prosperi R, Omodei D, Prakash N, Wurst W, Simeone A
Nature neuroscience 2010 Dec;13(12):1481-8
Nature neuroscience 2010 Dec;13(12):1481-8
Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
FEBS letters 2009 Feb 4;583(3):561-7
FEBS letters 2009 Feb 4;583(3):561-7
Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
Stem cells (Dayton, Ohio) 2007 Jun;25(6):1511-20
Stem cells (Dayton, Ohio) 2007 Jun;25(6):1511-20
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Otx2 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Otx2 by Simple WesternTM. Simple Western lane view shows lysates of IMR-32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Otx2 in NTera-2 Human Cell Line. Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Otx2 in Mouse Embryo. Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.