BAF1979

antibody from R&D Systems

Targeting: OTX2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF1979

Provider

R&D Systems

Product name

Human Otx2 Biotinylated Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Otx2 in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Biotin

Antigen sequence

P32243


Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Synchrony and asynchrony between an epigenetic clock and developmental timing.

Hoshino A, Horvath S, Sridhar A, Chitsazan A, Reh TA
Scientific reports 2019 Mar 6;9(1):3770

---

RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion.

Fairchild CL, Hino K, Han JS, Miltner AM, Peinado Allina G, Brown CE, Burns ME, La Torre A, Simó S
Development (Cambridge, England) 2018 Feb 2;145(3)

---

Combinatorial regulation of a Blimp1 (Prdm1) enhancer in the mouse retina.

Mills TS, Eliseeva T, Bersie SM, Randazzo G, Nahreini J, Park KU, Brzezinski JA 4th
PloS one 2017;12(8):e0176905

---

Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa.

Sharma TP, Wiley LA, Whitmore SS, Anfinson KR, Cranston CM, Oppedal DJ, Daggett HT, Mullins RF, Tucker BA, Stone EM
Stem cell research 2017 May;21:58-70

---

Arap1 Deficiency Causes Photoreceptor Degeneration in Mice.

Moshiri A, Humpal D, Leonard BC, Imai DM, Tham A, Bower L, Clary D, Glaser TM, Lloyd KC, Murphy CJ
Investigative ophthalmology & visual science 2017 Mar 1;58(3):1709-1718

---

Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina.

Park KU, Randazzo G, Jones KL, Brzezinski JA 4th
Investigative ophthalmology & visual science 2017 Feb 1;58(2):1137-1150

---

cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness.

Wiley LA, Burnight ER, DeLuca AP, Anfinson KR, Cranston CM, Kaalberg EE, Penticoff JA, Affatigato LM, Mullins RF, Stone EM, Tucker BA
Scientific reports 2016 Jul 29;6:30742

---

The GIPC1-Akt1 Pathway Is Required for the Specification of the Eye Field in Mouse Embryonic Stem Cells.

La Torre A, Hoshino A, Cavanaugh C, Ware CB, Reh TA
Stem cells (Dayton, Ohio) 2015 Sep;33(9):2674-85

---

A transient wave of BMP signaling in the retina is necessary for Müller glial differentiation.

Ueki Y, Wilken MS, Cox KE, Chipman LB, Bermingham-McDonogh O, Reh TA
Development (Cambridge, England) 2015 Feb 1;142(3):533-43

---

Stage-dependent requirement of neuroretinal Pax6 for lens and retina development.

Klimova L, Kozmik Z
Development (Cambridge, England) 2014 Mar;141(6):1292-302

---

Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation.

Tucker BA, Anfinson KR, Mullins RF, Stone EM, Young MJ
Stem cells translational medicine 2013 Jan;2(1):16-24

---

Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence.

Brzezinski JA 4th, Uoon Park K, Reh TA
Developmental biology 2013 Dec 15;384(2):194-204

---

Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region.

Achim K, Peltopuro P, Lahti L, Li J, Salminen M, Partanen J
Development (Cambridge, England) 2012 Jul;139(13):2360-70

---

OTX2 is critical for the maintenance and progression of Shh-independent medulloblastomas.

Adamson DC, Shi Q, Wortham M, Northcott PA, Di C, Duncan CG, Li J, McLendon RE, Bigner DD, Taylor MD, Yan H
Cancer research 2010 Jan 1;70(1):181-91

---

Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development.

Brzezinski JA 4th, Lamba DA, Reh TA
Development (Cambridge, England) 2010 Feb;137(4):619-29

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Otx2 in BG01V Human Embryonic Stem Cells. Otx2 was detected in immersion fixed BG01V human embryonic stem cells differentiated to ectoderm using Goat Anti-Human Otx2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated AntI-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.