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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-51246 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGAM5 Recombinant Rabbit Monoclonal Antibody (7J3A5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GARPGPGVWD PNWDRREPLS LINVRKRNVE SGEEELASKL DHYKAKATRH IFLIRHSQYH VDGSLEKDRT LTPLGREQAE LTGLRLASLG LKFNKIV
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 7J3A5
- Vial size
- 100 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry (confocal imaging) analysis of Hep G2 cells using PGAM5 Recombinant monoclonal antibody (Product # MA5-51246) using a dilution of 1:300 (Red). The cells were counterstained with alpha-Tubulin monoclonal antibody (Product # MA5-38221) using a dilution of 1:400 (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGAM5 in paraffin-embedded human liver cancer. Samples were incubated with PGAM5 Recombinant monoclonal antibody (Product # MA5-51246) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGAM5 in paraffin-embedded human thyroid cancer. Samples were incubated with PGAM5 Recombinant monoclonal antibody (Product # MA5-51246) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
