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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- AM02221PU-N - Provider product page
- Provider
- OriGene
- Product name
- DEFB4B (4-41) mouse monoclonal antibody, clone L12-4C-C2, Purified
- Antibody type
- Monoclonal
- Description
- DEFB4B (4-41) mouse monoclonal antibody, clone L12-4C-C2, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DEFB4B
- Isotype
- IgG
- Antibody clone number
- L12-4C-C2
- Vial size
- 100 µg
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Supportive validation
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- OriGene (provider)
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- Experimental details
- Immunohistochemical staining of Beta-Defensin-2 in Paraffin Section of Human colon resection tissue. Antigen retrieval was performed in citric acid (0.01M, pH6) twice for 7 min and in 0.1% Trypsin for 5 min. The section was incubated with AM02221PU (1/2000) for 1 h and detected using Alkaline Phophatase anti-Alkaline Phophatase method. New fuchsin was used as the chromogen. The section was counterstained with hematoxylin. AM02221PU stains colonic epithelial enterocytes in ulcerative colitis patients. Langhorst J et al. (2009) Am. J. Gastroenterol 104(2): 404-410.
- Validation comment
- IHC
