702736

antibody from Invitrogen Antibodies

Targeting: EMC3 TMEM111

Western blot

Antibody data

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Validation data

Product number: 702736 - Provider product page
Provider: Invitrogen Antibodies
Product name: TM111 Recombinant Rabbit Monoclonal Antibody (3H4L5)
Antibody type: Monoclonal
Antigen: Other
Description: This antibody is predicted to react with Monkey, Horse, Cat, Mouse Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Antibody clone number: 3H4L5
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

EMC3 protein structure - 702736 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: For immunofluorescence analysis, HepG2 cells were fixed for detection of endogenous EMC3 using Anti- EMC3 Recombinant Rabbit Monoclonal Antibody (Product # 702736, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EMC3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of EMC3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Emc3 in NIH3T3 ectopically expressing Myc-tagged EMC3 cells. The cells were fixed with 4% PFA for 10 min, permeabilized with 0.2% Triton X-100 for 15 min, and blocked with blocking buffer (5% normal donkey serum in 0.02% Triton X-100/PBS) for 1hr at room temperature. Cells were stained with a Emc3 monoclonal antibody (Product # 702736, red) at a dilution of 1:100 in blocking buffer for 1.5 hours at room temperature. Ectopic expression of myc-tagged Emc3 was also detected by a c-myc antibody (green). a-tubulin and DAPI stainings were shown in purple and blue respectively. Data courtesy of Dr. Jeff Whitsett's Lab at Cincinnati Children's Hospital Medical Center.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Emc3 in NIH3T3 cells. The cells were fixed with 4% PFA for 10 min, permeabilized with 0.2% Triton X-100 for 15 min, and blocked with blocking buffer (5% normal donkey serum in 0.02% Triton X-100/PBS) for 1hr at room temperature. Cells were stained with a Emc3 monoclonal antibody (Product # 702736, red) at a dilution of 1:100 in blocking buffer for 1.5 hours at room temperature. a-tubulin and DAPI stainings were shown in purple and blue respectively. Data courtesy of Dr. Jeff Whitsett's Lab at Cincinnati Children's Hospital Medical Center.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Emc3 in NIH3T3 ectopically expressing Myc-tagged EMC3 cells. The cells were fixed with 4% PFA for 10 min, permeabilized with 0.2% Triton X-100 for 15 min, and blocked with blocking buffer (5% normal donkey serum in 0.02% Triton X-100/PBS) for 1hr at room temperature. Cells were stained with a Emc3 monoclonal antibody (Product # 702736, red) at a dilution of 1:100 in blocking buffer for 1.5 hours at room temperature. Ectopic expression of myc-tagged Emc3 was also detected by a c-myc antibody (green). a-tubulin and DAPI stainings were shown in purple and blue respectively. Data courtesy of Dr. Jeff Whitsett's Lab at Cincinnati Children's Hospital Medical Center.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Emc3 in NIH3T3 cells. The cells were fixed with 4% PFA for 10 min, permeabilized with 0.2% Triton X-100 for 15 min, and blocked with blocking buffer (5% normal donkey serum in 0.02% Triton X-100/PBS) for 1hr at room temperature. Cells were stained with a Emc3 monoclonal antibody (Product # 702736, red) at a dilution of 1:100 in blocking buffer for 1.5 hours at room temperature. a-tubulin and DAPI stainings were shown in purple and blue respectively. Data courtesy of Dr. Jeff Whitsett's Lab at Cincinnati Children's Hospital Medical Center.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: For immunofluorescence analysis, HepG2 cells were fixed for detection of endogenous EMC3 using Anti- EMC3 Recombinant Rabbit Monoclonal Antibody (Product # 702736, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EMC3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of EMC3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.