Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB4905-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Siglec-14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human Siglec-14 in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q08ET2
- Isotype
- IgG
- Antibody clone number
- 2457E
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Siglec-14 in HEK293 Human Cell Line Transfected with Human Siglec-14 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Human Siglec-14 (filled histogram) or irrelevant protein (open histogram) were stained with Rabbit Anti-Human Siglec-14 Monoclonal Antibody (Catalog # MAB4905) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Gate was set based on Rabbit IgG isotype control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.