A305-868A-T

antibody from Invitrogen Antibodies

Targeting: ANKRD13A ANKRD13, NY-REN-25

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-868A-T

Provider

Invitrogen Antibodies

Product name

ANKRD13A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse ANKRD13A by western blot. Samples: Whole cell lysate (15 µg) from Jurkat, GaMG, HEK293T, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-ANKRD13A antibody A305-868A-M (lot A305-868A-M-1) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ANKRD13A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-ANKRD13A antibody (Product # A305-868A-T; lot A305-868A-T-1) used for IP at 100 µL per reaction. ANKRD13A was also immunoprecipitated by rabbit anti-ANKRD13A antibody (Product # A305-869A-T). For blotting immunoprecipitated ANKRD13A, Product # A305-868A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 seconds.