Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-18496 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGRMC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 4,000.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Liver lysate using Product # PA5-18496 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HEK293 overexpressing Human PGRMC1 using Product # PA5-18496, mock transfection as a control in first lane.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KD of PGRMC1 was achieved by transfecting NTERA-2 with PGRMC1 specific siRNAs (Silencer® select Product S21310). Western blot analysis (Fig. a) was performed using whole cell extracts from the PGRMC1 KD cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PGRMC1 Polyclonal Antibody (Product # PA5-18496, 0.1µg/mL) and detected by chemiluminescence Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27014, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PGRMC1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PGRMC1 Goat Polyclonal Antibody (Product # PA5-18496) and 21 kDa band corresponding to PGRMC1 was observed along with uncharacterized bands at 40 kDa and 60 kDa. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), NTERA-2 (Lane 2) and tissue extract of Mouse Liver (Lane 3) were electrophoresed using Novex® NuPAGE® 12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/mL) and detected by Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PGRMC1 in Human Testis using a PGRMC1 monoclonal antibody (Product #PA5-18496) at 5 µg/mL. The Human Testis tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.