PA5-57265

antibody from Invitrogen Antibodies

Targeting: METTL8 FLJ13984, TIP

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57265

Provider

Invitrogen Antibodies

Product name

METTL8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: MNMIWRNSIS CLRLGKVPHR YQSGYHPVAP LGSRILTDPA KVFEHNMWDH MQWSKEEEAA ARKKVKENSA VRVLLEE Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 82%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.34 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Methyltransferase METTL8 is required for 3-methylcytosine modification in human mitochondrial tRNAs.

Lentini JM, Bargabos R, Chen C, Fu D
The Journal of biological chemistry 2022 Apr;298(4):101788

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of METTL8 in human colon using METTL8 Polyclonal Antibody (Product # PA5-57265) shows weak cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of METTL8 in human kidney using METTL8 Polyclonal Antibody (Product # PA5-57265) shows moderate cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of METTL8 in human stomach using METTL8 Polyclonal Antibody (Product # PA5-57265) shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of METTL8 in human testis using METTL8 Polyclonal Antibody (Product # PA5-57265) shows weak cytoplasmic positivity in Leydig cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 METTL8 is required for efficient m 3 C formation in mitochondrial tRNAs in vivo . A , CRISPR/Cas9 gene KO strategy depicting sequence guide RNAs (sgRNAs) targeting exon 3 of the human METTL8 gene. B , genomic PCR demonstrating the loss of exon 3 in METTL8-KO cell lines. Deletion of exon 3 results in a 72 base-pair deletion. C , immunoblot analysis of METTL8 expression in WT versus METTL8-KO human cell lines. Actin was used as a loading control. D , (i) schematic of the Positive Hybridization in the Absence of Modification (PHA) to monitor m 3 C status. PHA probe spans position C32 while the control probe spans a region lacking modifications on the same tRNA. (ii) schematic of primer extension assay to monitor m 3 C modification. E , Northern blot analysis using the PHA assay with probes designed to detect m 3 C at position 32 and a control probe that hybridizes to a different area of the same tRNA. PHA quantification represents the ratio of PHA versus control probe signal expressed relative to the WT1 cell line. Northern blotting and quantification were performed on three independent cell preparations for each cell line with comparable results. F , representative gel from primer extension assays to monitor the presence of m 3 C in mt-tRNA-Thr-UGU from the indicated cell lines. G , quantification of m 3 C formation in ( F ). Primer extension analysis was repeated three times and bars represent the standard deviation from the mean. METTL8, methyltransferase-like 8; mt,