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Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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Validation data
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- Product number
- A305-744A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAPK1IP1L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MAPK1IP1L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAPK1IP1L antibody (Product # A305-744A-T (lot A305-744A-T-2) used for IP at 15 µL per reaction. MAPK1IP1L was also immunoprecipitated by a previous lot of this antibody (lot A305-744A-T-1) and rabbit anti-MAPK1IP1L antibodies (Product # A305-743A-T and A305-745A-T). For blotting immunoprecipitated MAPK1IP1L, Product # A305-743A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MAPK1IP1L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAPK1IP1L antibody A305-744A-M (lot A305-744A-M-1) used for IP at 8 µl per reaction. MAPK1IP1L was also immunoprecipitated by rabbit anti-MAPK1IP1L antibody A305-745A-M. For blotting immunoprecipitated MAPK1IP1L, A305-744A-M was used at 1:4000. Detection: Chemiluminescence with an exposure time of 30 seconds.
