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LearnMA5-55913
antibody from Invitrogen Antibodies
Targeting: PPP1R14D
CPI17-like, FLJ20251, GBPI-1, MGC119014, MGC119016
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-55913 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP1R14D Monoclonal Antibody (K49008_16C7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MLSSSPASCT SPSPDGENPC KKVHWASGRR RTSSTDSESK SHPDSSKIPR SRRPSRLTVK YDRGQLQRWL EMEQWVDAQV QELFQDQATP SEPEIDLEAL MDLSTEEQKT QLEAILGNCP RPTEAFISEL LSQLKKLRRL SRPQK
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- K49008_16C7
- Vial size
- 50 μg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PPP1R14D in paraffin-embedded colon cancer tissue. Sample was incubated with PPP1R14D monoclonal antibody (Product # MA5-55913) at a dilution of 5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin.
