Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-36305 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MID1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 75 kDa.
- Concentration
- 1 mg/mL
Submitted references TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease.
Chen Q, Gao C, Wang M, Fei X, Zhao N
Frontiers in physiology 2021;12:709506
Frontiers in physiology 2021;12:709506
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MID1 in Lane 1: A549 whole cell lysate (40 µg), Lane 2: HEK293T whole cell lysate (40 µg), Lane 3: 786-O whole cell lysate (40 µg), Lane 4: L02 whole cell lysate (40 µg), Lane 5: HepG2 whole cell lysate (40 µg). Samples were incubated with MID1 polyclonal antibody (Product # PA5-36305) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Midline-1 using Midline-1 polyclonal antibody (Product # PA5-36305) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: PC12 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MID1 in paraffin-embedded human tonsil carcinoma tissue. Samples were incubated with MID1 polyclonal antibody (Product # PA5-36305) at a dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Midline-1 in paraffin-embedded human tonsil carcinoma using Midline-1 polyclonal antibody (Product # PA5-36305) at a dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 TRIM18 interacts with protein tyrosine phosphatases (PTP1B) and promotes PTP1B ubiquitination. (A) HK-2 cells were treated with HG and transduced with indicated vectors, and then TRIM18, SHP-1, SOCS1, PTP1B, and PIAS1 expression was measured. (B,C) Cell lysates were immunoprecipitated and then blotted with the indicated antibodies. (D-F) Cells were transduced with indicated vectors with/without 10 muM MG132, and then PTP1B expression was measured. (G) PTP1B was immunoprecipitated and immunoblotted. Values are presented as mean +- SD. n = 3/group. *** p < 0.001 vs. shNC or vector+vehicle. ### p < 0.001 vs. oeTRIM18+vehicle.
