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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-36305 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MID1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 75 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease.
Chen Q, Gao C, Wang M, Fei X, Zhao N
Frontiers in physiology 2021;12:709506
Frontiers in physiology 2021;12:709506
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 5 TRIM18 interacts with protein tyrosine phosphatases (PTP1B) and promotes PTP1B ubiquitination. (A) HK-2 cells were treated with HG and transduced with indicated vectors, and then TRIM18, SHP-1, SOCS1, PTP1B, and PIAS1 expression was measured. (B,C) Cell lysates were immunoprecipitated and then blotted with the indicated antibodies. (D-F) Cells were transduced with indicated vectors with/without 10 muM MG132, and then PTP1B expression was measured. (G) PTP1B was immunoprecipitated and immunoblotted. Values are presented as mean +- SD. n = 3/group. *** p < 0.001 vs. shNC or vector+vehicle. ### p < 0.001 vs. oeTRIM18+vehicle.
