16413-1-AP

antibody from Proteintech Group

Targeting: MIEF2 MGC23130, MiD49, SMCR7

Provider product page for 16413-1-AP

Western blot

ELISA

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [53]
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Validations
Immunohistochemistry [1]

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Product number: 16413-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16413-1-AP, RRID:AB_2714217
Product name: SMCR7/MID49 antibody
Antibody type: Polyclonal
Description: KD/KO validated SMCR7/MID49 antibody (Cat. #16413-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MIEF2 protein structure - 16413-1-AP shown in red.

Submitted references The Footprints of Mitochondrial Fission and Apoptosis in Fluoride-Induced Renal Dysfunction.

Zuo Q, Lin L, Zhang Y, Ommati MM, Wang H, Zhao J
Biological trace element research 2024 Sep;202(9):4125-4135

MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA levels.

Tábara LC, Burr SP, Frison M, Chowdhury SR, Paupe V, Nie Y, Johnson M, Villar-Azpillaga J, Viegas F, Segawa M, Anand H, Petkevicius K, Chinnery PF, Prudent J
Cell 2024 Jul 11;187(14):3619-3637.e27

MTCH2 cooperates with MFN2 and lysophosphatidic acid synthesis to sustain mitochondrial fusion.

Goldman A, Mullokandov M, Zaltsman Y, Regev L, Levin-Zaidman S, Gross A
EMBO reports 2024 Jan;25(1):45-67

The mystery of phospho-Drp1 with four adaptors in cell cycle: when mitochondrial fission couples to cell fate decisions.

Wu NS, Ma IC, Lin YF, Ko HJ, Loh JK, Hong YR
Cell cycle (Georgetown, Tex.) 2023 Nov;22(21-22):2485-2503

Mitochondrial E3 ubiquitin ligase MARCHF5 controls BAK apoptotic activity independently of BH3-only proteins.

Huang AS, Chin HS, Reljic B, Djajawi TM, Tan IKL, Gong JN, Stroud DA, Huang DCS, van Delft MF, Dewson G
Cell death and differentiation 2023 Mar;30(3):632-646

Chemical inhibition of mitochondrial fission via targeting the DRP1-receptor interaction.

Yang J, Chen P, Cao Y, Liu S, Wang W, Li L, Li J, Jiang Z, Ma Y, Chen S, Zheng S, Qi X, Jiang H
Cell chemical biology 2023 Mar 16;30(3):278-294.e11

Senomorphic effect of diphenyleneiodonium through AMPK/MFF/DRP1 mediated mitochondrial fission.

Liao KM, Chen CJ, Luo WJ, Hsu CW, Yu SL, Yang PC, Su KY
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Jun;162:114616

UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy.

Zheng J, Cao Y, Yang J, Jiang H
EMBO reports 2022 Oct 6;23(10):e54859

AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology.

Tilokani L, Russell FM, Hamilton S, Virga DM, Segawa M, Paupe V, Gruszczyk AV, Protasoni M, Tabara LC, Johnson M, Anand H, Murphy MP, Hardie DG, Polleux F, Prudent J
Science advances 2022 Nov 11;8(45):eabo7956

Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis.

Carter RJ, Milani M, Beckett AJ, Liu S, Prior IA, Cohen GM, Varadarajan S
Cell death & disease 2022 May 4;13(5):436

Myosin II proteins are required for organization of calcium-induced actin networks upstream of mitochondrial division.

Kage F, Vicente-Manzanares M, McEwan BC, Kettenbach AN, Higgs HN
Molecular biology of the cell 2022 Jun 1;33(7):ar63

Cdk5 Promotes Mitochondrial Fission via Drp1 Phosphorylation at S616 in Chronic Ethanol Exposure-Induced Cognitive Impairment.

Liu D, Li J, Rong X, Li J, Peng Y, Shen Q
Molecular neurobiology 2022 Dec;59(12):7075-7094

King WT, Axelrod CL, Zunica ERM, Noland RC, Davuluri G, Fujioka H, Tandler B, Pergola K, Hermann GE, Rogers RC, López-Domènech S, Dantas WS, Stadler K, Hoppel CL, Kirwan JP
The Journal of biological chemistry 2021 Oct;297(4):101196

Chaperone-mediated autophagy controls the turnover of E3 ubiquitin ligase MARCHF5 and regulates mitochondrial dynamics.

Nie T, Tao K, Zhu L, Huang L, Hu S, Yang R, Xu P, Mao Z, Yang Q
Autophagy 2021 Oct;17(10):2923-2938

ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.

Lin BC, Phung TH, Higgins NR, Greenslade JE, Prado MA, Finley D, Karbowski M, Polster BM, Monteiro MJ
Human molecular genetics 2021 Jun 17;30(13):1230-1246

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

König T, Nolte H, Aaltonen MJ, Tatsuta T, Krols M, Stroh T, Langer T, McBride HM
Nature cell biology 2021 Dec;23(12):1271-1286

Lipids activate skeletal muscle mitochondrial fission and quality control networks to induce insulin resistance in humans.

Axelrod CL, Fealy CE, Erickson ML, Davuluri G, Fujioka H, Dantas WS, Huang E, Pergola K, Mey JT, King WT, Mulya A, Hsia D, Burguera B, Tandler B, Hoppel CL, Kirwan JP
Metabolism: clinical and experimental 2021 Aug;121:154803

Mst1 Knockout Alleviates Mitochondrial Fission and Mitigates Left Ventricular Remodeling in the Development of Diabetic Cardiomyopathy.

Feng X, Wang S, Yang X, Lin J, Man W, Dong Y, Zhang Y, Zhao Z, Wang H, Sun D
Frontiers in cell and developmental biology 2020;8:628842

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Nagashima S, Tábara LC, Tilokani L, Paupe V, Anand H, Pogson JH, Zunino R, McBride HM, Prudent J
Science (New York, N.Y.) 2020 Mar 20;367(6484):1366-1371

Mitochondrial Dynamics Regulation in Skin Fibroblasts from Mitochondrial Disease Patients.

Tokuyama T, Hirai A, Shiiba I, Ito N, Matsuno K, Takeda K, Saito K, Mii K, Matsushita N, Fukuda T, Inatome R, Yanagi S
Biomolecules 2020 Mar 13;10(3)

NME3 Regulates Mitochondria to Reduce ROS-Mediated Genome Instability.

Chen CW, Tsao N, Zhang W, Chang ZF
International journal of molecular sciences 2020 Jul 17;21(14)

Drp1/Mff signaling pathway is involved in fluoride-induced abnormal fission of hepatocyte mitochondria in mice.

Zhou BH, Wei SS, Jia LS, Zhang Y, Miao CY, Wang HW
The Science of the total environment 2020 Jul 10;725:138192

The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity.

Yasukawa K, Kinoshita D, Yaku K, Nakagawa T, Koshiba T
The Journal of biological chemistry 2020 Jan 10;295(2):444-457

Mitochondrial Safeguard: a stress response that offsets extreme fusion and protects respiratory function via flickering-induced Oma1 activation.

Murata D, Yamada T, Tokuyama T, Arai K, Quirós PM, López-Otín C, Iijima M, Sesaki H
The EMBO journal 2020 Dec 15;39(24):e105074

MARCH5 requires MTCH2 to coordinate proteasomal turnover of the MCL1:NOXA complex.

Djajawi TM, Liu L, Gong JN, Huang AS, Luo MJ, Xu Z, Okamoto T, Call MJ, Huang DCS, van Delft MF
Cell death and differentiation 2020 Aug;27(8):2484-2499

Cardiac Mitochondrial PTEN-L determines cell fate between apoptosis and survival during chronic alcohol consumption.

Sivakumar A, Shanmugarajan S, Subbiah R, Balakrishnan R
Apoptosis : an international journal on programmed cell death 2020 Aug;25(7-8):590-604

Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.

Ma D, Zheng B, Liu HL, Zhao YB, Liu X, Zhang XH, Li Q, Shi WB, Suzuki T, Wen JK
PLoS biology 2020 Aug;18(8):e3000808

Downregulation of MiD49 contributes to tumor growth and metastasis of human pancreatic cancer.

Bai L, Liang J, Li L, Li E
Oncology reports 2020 Apr;43(4):1208-1220

An epigenetic increase in mitochondrial fission by MiD49 and MiD51 regulates the cell cycle in cancer: Diagnostic and therapeutic implications.

Dasgupta A, Chen KH, Wu D, Hoskin V, Mewburn J, Lima PDA, Parlow LRG, Hindmarch CCT, Martin A, Sykes EA, Tayade C, Lightbody ED, Madarnas Y, SenGupta SK, Elliott BE, Nicol CJB, Archer SL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Apr;34(4):5106-5127

Huangqi-Danshen Decoction Ameliorates Adenine-Induced Chronic Kidney Disease by Modulating Mitochondrial Dynamics.

Liu X, Huang S, Wang F, Zheng L, Lu J, Chen J, Li S
Evidence-based complementary and alternative medicine : eCAM 2019;2019:9574045

Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff).

Lee L, Seager R, Nakamura Y, Wilkinson KA, Henley JM
PloS one 2019;14(5):e0213116

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Xian H, Yang Q, Xiao L, Shen HM, Liou YC
Nature communications 2019 May 3;10(1):2059

Apogossypol-mediated reorganisation of the endoplasmic reticulum antagonises mitochondrial fission and apoptosis.

Yedida G, Milani M, Cohen GM, Varadarajan S
Cell death & disease 2019 Jul 8;10(7):521

Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.

Chen CW, Wang HL, Huang CW, Huang CY, Lim WK, Tu IC, Koorapati A, Hsieh ST, Kan HW, Tzeng SR, Liao JC, Chong WM, Naroditzky I, Kidron D, Eran A, Nijim Y, Sela E, Feldman HB, Kalfon L, Raveh-Barak H, Falik-Zaccai TC, Elpeleg O, Mandel H, Chang ZF
Proceedings of the National Academy of Sciences of the United States of America 2019 Jan 8;116(2):566-574

Andrographolide alleviates Parkinsonism in MPTP-PD mice via targeting mitochondrial fission mediated by dynamin-related protein 1.

Geng J, Liu W, Gao J, Jiang C, Fan T, Sun Y, Qin ZH, Xu Q, Guo W, Gao J
British journal of pharmacology 2019 Dec;176(23):4574-4591

Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.

Xian H, Liou YC
Autophagy 2019 Dec;15(12):2107-2125

MITOL deletion in the brain impairs mitochondrial structure and ER tethering leading to oxidative stress.

Nagashima S, Takeda K, Ohno N, Ishido S, Aoki M, Saitoh Y, Takada T, Tokuyama T, Sugiura A, Fukuda T, Matsushita N, Inatome R, Yanagi S
Life science alliance 2019 Aug;2(4)

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Zhong X, Cui P, Cai Y, Wang L, He X, Long P, Lu K, Yan R, Zhang Y, Pan X, Zhao X, Li W, Zhang H, Zhou Q, Gao P
Cell metabolism 2019 Apr 2;29(4):979-992.e4

Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.

Xiong PY, Tian L, Dunham-Snary KJ, Chen KH, Mewburn JD, Neuber-Hess M, Martin A, Dasgupta A, Potus F, Archer SL
Frontiers in cardiovascular medicine 2018;5:195

Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005

A novel mechanism causing imbalance of mitochondrial fusion and fission in human myopathies.

Bartsakoulia M, Pyle A, Troncoso-Chandía D, Vial-Brizzi J, Paz-Fiblas MV, Duff J, Griffin H, Boczonadi V, Lochmüller H, Kleinle S, Chinnery PF, Grünert S, Kirschner J, Eisner V, Horvath R
Human molecular genetics 2018 Apr 1;27(7):1186-1195

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Wang L, Zhang T, Wang L, Cai Y, Zhong X, He X, Hu L, Tian S, Wu M, Hui L, Zhang H, Gao P
The EMBO journal 2017 May 15;36(10):1330-1347

Foxo3a inhibits mitochondrial fission and protects against doxorubicin-induced cardiotoxicity by suppressing MIEF2.

Zhou L, Li R, Liu C, Sun T, Htet Aung LH, Chen C, Gao J, Zhao Y, Wang K
Free radical biology & medicine 2017 Mar;104:360-370

Sirtuin 5 protects mitochondria from fragmentation and degradation during starvation.

Guedouari H, Daigle T, Scorrano L, Hebert-Chatelain E
Biochimica et biophysica acta. Molecular cell research 2017 Jan;1864(1):169-176

Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population.

Vannuvel K, Van Steenbrugge M, Demazy C, Ninane N, Fattaccioli A, Fransolet M, Renard P, Raes M, Arnould T
Journal of cellular physiology 2016 Sep;231(9):1913-31

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Li H, Ruan Y, Zhang K, Jian F, Hu C, Miao L, Gong L, Sun L, Zhang X, Chen S, Chen H, Liu D, Song Z
Cell death and differentiation 2016 Mar;23(3):380-92

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Wu W, Lin C, Wu K, Jiang L, Wang X, Li W, Zhuang H, Zhang X, Chen H, Li S, Yang Y, Lu Y, Wang J, Zhu R, Zhang L, Sui S, Tan N, Zhao B, Zhang J, Li L, Feng D
The EMBO journal 2016 Jul 1;35(13):1368-84

Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.

Korwitz A, Merkwirth C, Richter-Dennerlein R, Tröder SE, Sprenger HG, Quirós PM, López-Otín C, Rugarli EI, Langer T
The Journal of cell biology 2016 Jan 18;212(2):157-66

Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.

Xu S, Cherok E, Das S, Li S, Roelofs BA, Ge SX, Polster BM, Boyman L, Lederer WJ, Wang C, Karbowski M
Molecular biology of the cell 2016 Jan 15;27(2):349-59

Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.

Otera H, Miyata N, Kuge O, Mihara K
The Journal of cell biology 2016 Feb 29;212(5):531-44

The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission.

Anand R, Wai T, Baker MJ, Kladt N, Schauss AC, Rugarli E, Langer T
The Journal of cell biology 2014 Mar 17;204(6):919-29

Loss of Yme1L perturbates mitochondrial dynamics.

Ruan Y, Li H, Zhang K, Jian F, Tang J, Song Z
Cell death & disease 2013 Oct 31;4(10):e896

Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission.

Losón OC, Song Z, Chen H, Chan DC
Molecular biology of the cell 2013 Mar;24(5):659-67

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The SMCR7,MID49 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SMCR7,MID49. This antibody recognizes human,mouse,rat antigen. The SMCR7,MID49 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 16413-1-AP.pdf

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