PA5-71433

antibody from Invitrogen Antibodies

Targeting: AMER2 FAM123A, FLJ25477

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-71433

Provider

Invitrogen Antibodies

Product name

AMER2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Exosomal miR-128-3p Promotes Epithelial-to-Mesenchymal Transition in Colorectal Cancer Cells by Targeting FOXO4 via TGF-β/SMAD and JAK/STAT3 Signaling.

Bai J, Zhang X, Shi D, Xiang Z, Wang S, Yang C, Liu Q, Huang S, Fang Y, Zhang W, Song J, Xiong B
Frontiers in cell and developmental biology 2021;9:568738

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Characterization of exosomes derived from CRC cells. (A) TEM images showing small vesicles of ~40-100 nm in diameter, scale bar = 100 nm. (B) Western blot characterization of exosomal markers CD63, CD81, and TSG101 in isolated exosomes and cell lysates after extraction. (C) Heat map showing the changes in miRNA expression in exosomes isolated from non-induced (Con) and IL-6-induced HCT-116 cells. (D) qRT-PCR of exosomal miRNA expression in non-induced (Con) and IL-6-induced HCT-116 cells. (E) qRT-PCR and (F) western blot of the expression of miR-128-3p target genes FOXO4, SOCS5, AMER2, HIC1, and SMURF2 in non-induced (Con) and IL-6-induced HCT-116 cells. Protein expression was normalized to that of GAPDH. (G) Relative luciferase activity of reporter plasmids carrying WT or MUT FOXO4 3'-UTR in HCT-116 cells co-transfected with miR-128-3p mimics or its negative control (miR-NC). (H) Decay curve representative of FOXO4 mRNA degradation in HCT-116 cells carrying miR-128-3p mimics or mimic-NC. Data are shown as the mean +- SD ( n = 3); * P < 0.05.