Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-71433 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMER2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Exosomal miR-128-3p Promotes Epithelial-to-Mesenchymal Transition in Colorectal Cancer Cells by Targeting FOXO4 via TGF-β/SMAD and JAK/STAT3 Signaling.
Bai J, Zhang X, Shi D, Xiang Z, Wang S, Yang C, Liu Q, Huang S, Fang Y, Zhang W, Song J, Xiong B
Frontiers in cell and developmental biology 2021;9:568738
Frontiers in cell and developmental biology 2021;9:568738
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMER2 on human mesenchymoma tumor cells. The sample was probed with a AMER2 polyclonal antibody (Product # PA5-71433) using a primary antibody dilution of 1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Characterization of exosomes derived from CRC cells. (A) TEM images showing small vesicles of ~40-100 nm in diameter, scale bar = 100 nm. (B) Western blot characterization of exosomal markers CD63, CD81, and TSG101 in isolated exosomes and cell lysates after extraction. (C) Heat map showing the changes in miRNA expression in exosomes isolated from non-induced (Con) and IL-6-induced HCT-116 cells. (D) qRT-PCR of exosomal miRNA expression in non-induced (Con) and IL-6-induced HCT-116 cells. (E) qRT-PCR and (F) western blot of the expression of miR-128-3p target genes FOXO4, SOCS5, AMER2, HIC1, and SMURF2 in non-induced (Con) and IL-6-induced HCT-116 cells. Protein expression was normalized to that of GAPDH. (G) Relative luciferase activity of reporter plasmids carrying WT or MUT FOXO4 3'-UTR in HCT-116 cells co-transfected with miR-128-3p mimics or its negative control (miR-NC). (H) Decay curve representative of FOXO4 mRNA degradation in HCT-116 cells carrying miR-128-3p mimics or mimic-NC. Data are shown as the mean +- SD ( n = 3); * P < 0.05.