LS-B11284

antibody from LSBio

Targeting: STAG2 SA-2, SA2, SCC3B

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B11284

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B11284, RRID:AB_2725802

Product name

IHC-plus™ STAG2 Antibody (aa1100-1150) LS-B11284

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human SA2 by Western Blot. Sample: Nuclear extract from ~500000 HeLa cells. Antibody: Affinity purified goat anti-SA2 used at 0.5 ug/ml. Detection: Chemiluminescence with 15 sec. exposure.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human and mouse SA2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SA2 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemical Localization of Human SA2. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA2 used at 1 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemical Localization of Human SA2. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA2 used at 1 ug/ml.