GTX129876

antibody from GeneTex

Targeting: STAG2 SA-2, SA2, SCC3B

Immunocytochemistry

Immunohistochemistry

Antibody data

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Validation data

STAG2 protein structure - GTX129876 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX129876) diluted at 1:2000.
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41836

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: STAG2 antibody detects STAG2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG2 antibody (GTX129876) diluted at a dilution of 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX129876) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX129876) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX129876) diluted at 1:2000.