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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX130299 - Provider product page
- Provider
- GeneTex
- Product name
- SA2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130299) diluted at 1:6000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STAG2 antibody detects STAG2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG2 antibody (GTX130299) diluted at a dilution of 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130299) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130299) diluted at 1:6000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SA2 antibody detects SA2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SA2 protein stained by SA2 antibody (GTX130299) diluted at 1:500.Red: Phalloidin, a F-actin marker, diluted at 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SA2 protein from HeLa whole cell extracts using 5 £gg of SA2 antibody (GTX130299).Western blot analysis was performed using SA2 antibody (GTX130299).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
