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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-40130 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STAG2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLa, and HepG2 cells. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.15 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Stromal antigen 2 in various whole cell extracts (30 µg). Samples were separated by 5% SDS-PAGE and the membrane was probed with a Stromal antigen 2 polyclonal antibody (Product # PA5-40130) at a dilution of 1:1000
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STAG2 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a STAG2 Polyclonal Antibody (Product # PA5-40130) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- STAG2 Polyclonal Antibody detects SA2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SA2 protein stained by STAG2 Polyclonal Antibody (Product # PA5-40130) diluted at 1:500.Red: Phalloidin, a F-actin marker, diluted at 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of SA2 was performed in HeLa whole cell extracts using 5 µg of STAG2 Polyclonal Antibody (Product # PA5-40130). Samples were transferred to a membrane and probed with STAG2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
