702499

antibody from Invitrogen Antibodies

Targeting: STAG2 SA-2, SA2, SCC3B

Western blot

Antibody data

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Validation data

Product number: 702499 - Provider product page
Provider: Invitrogen Antibodies
Product name: STAG2 Recombinant Rabbit Monoclonal Antibody (1H3L8)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Reactivity: Human
Host: Rabbit
Isotype: IgG
Antibody clone number: 1H3L8
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

STAG2 protein structure - 702499 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous STAG2 using Anti- STAG2 Recombinant Rabbit Monoclonal Antibody (Product # 702499, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of STAG2 (green) in the nucleus and cytoplasm can be clearly observed in interphase cells (a-d). When cell cycle progresses to prophase (e-h) STAG2 can be visualized detaching from chromatin and further progression to metaphase (i-l) and early cytokinesis (m-p) results in complete detachment of STAG2 from chromatin and translocation to cytoplasm.The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of STAG2 was achieved by transfecting HeLa cells with STAG2 specific siRNA (Silencer® select Product # s21089). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), and cells transfected with STAG2 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-STAG2 Recombinant Rabbit Monoclonal Antibody (Product # 702499, 2 µg/mL), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Loss of signal was observed upon siRNA mediated knockdown (panel i-l) confirming specificity of the antibody to STAG2 (green). The images were captured at 60X magnification.