PA5-78339

antibody from Invitrogen Antibodies

Targeting: STAG2 SA-2, SA2, SCC3B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78339

Provider

Invitrogen Antibodies

Product name

STAG2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, A431, HeLa, HepG2, Neuro2A, CD830, NIH-3T3, Raw264.7, C2C12 Predicted Reactivity: Rat (99%), Xenopus laevis (81%), Rabbit (98%), Bovine (99%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SA2 in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with SA2 polyclonal antibody (Product # PA5-78339) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SA2 in non-transfected (-) and transfected (+) 293T cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with SA2 polyclonal antibody (Product # PA5-78339) using a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SA2 in whole cell lysate using 50 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with SA2 polyclonal antibody (Product # PA5-78339) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using STAG2 Polyclonal Antibody (Product # PA5-78339). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG2 Polyclonal Antibody (Product # PA5-78339) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using STAG2 Polyclonal Antibody (Product # PA5-78339). Various whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG2 Polyclonal Antibody (Product # PA5-78339) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using STAG2 Polyclonal Antibody (Product # PA5-78339). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG2 Polyclonal Antibody (Product # PA5-78339) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using STAG2 Polyclonal Antibody (Product # PA5-78339). Various whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG2 Polyclonal Antibody (Product # PA5-78339) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of STAG2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: STAG2 Polyclonal Antibody (Product # PA5-78339) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of STAG2 was performed in paraffin-embedded human breast carcinoma tissue using STAG2 Polyclonal Antibody (Product # PA5-78339) at a dilution of 1:500.