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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [3]
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- Product number
- NB100-300 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-300, RRID:AB_10003416
- Product name
- Goat Polyclonal STAG2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Interaction of Sororin protein with polo-like kinase 1 mediates resolution of chromosomal arm cohesion.
Processing of lagging-strand intermediates in vitro by herpes simplex virus type 1 DNA polymerase.
Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells.
Cohesin associates with spindle poles in a mitosis-specific manner and functions in spindle assembly in vertebrate cells.
Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells.
A handcuff model for the cohesin complex.
Regulation of mitotic chromosome cohesion by Haspin and Aurora B.
Zhang N, Panigrahi AK, Mao Q, Pati D
The Journal of biological chemistry 2011 Dec 2;286(48):41826-37
The Journal of biological chemistry 2011 Dec 2;286(48):41826-37
Processing of lagging-strand intermediates in vitro by herpes simplex virus type 1 DNA polymerase.
Zhu Y, Wu Z, Cardoso MC, Parris DS
Journal of virology 2010 Aug;84(15):7459-72
Journal of virology 2010 Aug;84(15):7459-72
Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells.
Kong X, Mohanty SK, Stephens J, Heale JT, Gomez-Godinez V, Shi LZ, Kim JS, Yokomori K, Berns MW
Nucleic acids research 2009 May;37(9):e68
Nucleic acids research 2009 May;37(9):e68
Cohesin associates with spindle poles in a mitosis-specific manner and functions in spindle assembly in vertebrate cells.
Kong X, Ball AR Jr, Sonoda E, Feng J, Takeda S, Fukagawa T, Yen TJ, Yokomori K
Molecular biology of the cell 2009 Mar;20(5):1289-301
Molecular biology of the cell 2009 Mar;20(5):1289-301
Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells.
Yalcin S, Zhang X, Luciano JP, Mungamuri SK, Marinkovic D, Vercherat C, Sarkar A, Grisotto M, Taneja R, Ghaffari S
The Journal of biological chemistry 2008 Sep 12;283(37):25692-705
The Journal of biological chemistry 2008 Sep 12;283(37):25692-705
A handcuff model for the cohesin complex.
Zhang N, Kuznetsov SG, Sharan SK, Li K, Rao PH, Pati D
The Journal of cell biology 2008 Dec 15;183(6):1019-31
The Journal of cell biology 2008 Dec 15;183(6):1019-31
Regulation of mitotic chromosome cohesion by Haspin and Aurora B.
Dai J, Sullivan BA, Higgins JM
Developmental cell 2006 Nov;11(5):741-50
Developmental cell 2006 Nov;11(5):741-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAG2 Antibody [NB100-300] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAG2 Antibody [NB100-300] - Detection of Human SA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA2 antibody NB100-300 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAG2 Antibody [NB100-300] - Detection of human and mouse SA2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA2 antibody NB100-300 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
