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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 14-6525-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Synaptophysin Monoclonal Antibody (EP10), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This EP10 monoclonal antibody reacts with human Synaptophysin, which is also known as Major Synaptic Vesicle Protein p38. This 38-kDa integral membrane protein is expressed on neuronal presynaptic vesicles in the brain, spinal cord, retina, neuromuscular junctions, and adrenal medulla. Synaptophysin is also expressed in neuroendocrine tumors and neuroblastomas. Synaptophysin has been reported to play a role in synaptic vesicle trafficking. The EP10 antibody recognizes human and hamster synaptophysin but does not recognize mouse and rat synaptophysin. Applications Reported: This EP10 antibody has been reported for use in immunoprecipitation, immunoblotting (WB), immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry (ICC). Applications Tested: This EP10 antibody has been tested by immunohistochemistry on formalin fixed paraffin embedded human cerebellum using citrate buffer antigen retrieval. This antibody can be used at less than or equal to 5 µg/mL. This EP10 antibody has also been tested by western blot on human brain lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Hamster
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- EP10
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output.
Human neuroepithelial stem cell regional specificity enables spinal cord repair through a relay circuit.
Structure of synaptophysin: a hexameric MARVEL-domain channel protein.
Characterization of a synaptic antigen of interest in neuropsychiatric illness.
A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions.
Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles.
Giandomenico SL, Mierau SB, Gibbons GM, Wenger LMD, Masullo L, Sit T, Sutcliffe M, Boulanger J, Tripodi M, Derivery E, Paulsen O, Lakatos A, Lancaster MA
Nature neuroscience 2019 Apr;22(4):669-679
Nature neuroscience 2019 Apr;22(4):669-679
Human neuroepithelial stem cell regional specificity enables spinal cord repair through a relay circuit.
Dell'Anno MT, Wang X, Onorati M, Li M, Talpo F, Sekine Y, Ma S, Liu F, Cafferty WBJ, Sestan N, Strittmatter SM
Nature communications 2018 Aug 24;9(1):3419
Nature communications 2018 Aug 24;9(1):3419
Structure of synaptophysin: a hexameric MARVEL-domain channel protein.
Arthur CP, Stowell MH
Structure (London, England : 1993) 2007 Jun;15(6):707-14
Structure (London, England : 1993) 2007 Jun;15(6):707-14
Characterization of a synaptic antigen of interest in neuropsychiatric illness.
Honer WG, Kaufmann CA, Davies P
Biological psychiatry 1992 Jan 15;31(2):147-58
Biological psychiatry 1992 Jan 15;31(2):147-58
A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions.
Südhof TC, Lottspeich F, Greengard P, Mehl E, Jahn R
Science (New York, N.Y.) 1987 Nov 20;238(4830):1142-4
Science (New York, N.Y.) 1987 Nov 20;238(4830):1142-4
Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles.
Wiedenmann B, Franke WW
Cell 1985 Jul;41(3):1017-28
Cell 1985 Jul;41(3):1017-28
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Synaptophysin Monoclonal Antibody (EP10), eBioscience™ (Product # 14-6525-82) and a 38 kDa band corresponding to Synaptophysin was observed in SH-SY5Y and IMR-32, but not in SK-BR-3 and BJ. Whole cell extracts (40 µg lysate) of SH-SY5Y (Lane 1), IMR-32 (Lane 2), SK-BR-3 (Lane 3), BJ (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (5 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum using 5 µg/mL of Mouse IgG1 kappa Isotype Control (Product # 14-4714-82) (left) or Anti-Human Synaptophysin Purified (right) followed by Anti-Mouse IgG Biotin and DAB visualization.Nuclei are counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
