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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
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- Product number
- A05049 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Synaptophysin/SYP Picoband™ Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Synaptophysin/SYP detection. Tested with WB, ICC/IF in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Effects of spontaneous recurrent seizures on cognitive function via modulation of SNAREs expression.
The induction of cytochrome P450 2E1 by ethanol leads to the loss of synaptic proteins via PPARĪ± down-regulation.
Pentamethylquercetin protects against diabetes-related cognitive deficits in diabetic Goto-Kakizaki rats.
Downregulation of caveolin-1 contributes to the synaptic plasticity deficit in the hippocampus of aged rats.
Generation of retinal ganglion-like cells from reprogrammed mouse fibroblasts.
Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats.
Yu H, Yang X, Tang X, Tang R
The International journal of neuroscience 2018 Apr;128(4):376-383
The International journal of neuroscience 2018 Apr;128(4):376-383
The induction of cytochrome P450 2E1 by ethanol leads to the loss of synaptic proteins via PPARĪ± down-regulation.
Na S, Li J, Zhang H, Li Y, Yang Z, Zhong Y, Dong G, Yang J, Yue J
Toxicology 2017 Jun 15;385:18-27
Toxicology 2017 Jun 15;385:18-27
Pentamethylquercetin protects against diabetes-related cognitive deficits in diabetic Goto-Kakizaki rats.
Li XH, Xin X, Wang Y, Wu JZ, Jin ZD, Ma LN, Nie CJ, Xiao X, Hu Y, Jin MW
Journal of Alzheimer's disease : JAD 2013;34(3):755-67
Journal of Alzheimer's disease : JAD 2013;34(3):755-67
Downregulation of caveolin-1 contributes to the synaptic plasticity deficit in the hippocampus of aged rats.
Liu Y, Liang Z, Liu J, Zou W, Li X, Wang Y, An L
Neural regeneration research 2013 Oct 15;8(29):2725-33
Neural regeneration research 2013 Oct 15;8(29):2725-33
Generation of retinal ganglion-like cells from reprogrammed mouse fibroblasts.
Chen M, Chen Q, Sun X, Shen W, Liu B, Zhong X, Leng Y, Li C, Zhang W, Chai F, Huang B, Gao Q, Xiang AP, Zhuo Y, Ge J
Investigative ophthalmology & visual science 2010 Nov;51(11):5970-8
Investigative ophthalmology & visual science 2010 Nov;51(11):5970-8
Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats.
Liu J, He QJ, Zou W, Wang HX, Bao YM, Liu YX, An LJ
Brain research 2006 Dec 6;1123(1):68-79
Brain research 2006 Dec 6;1123(1):68-79
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of SYP using anti-SYP antibody (A05049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYP antigen affinity purified polyclonal antibody (Catalog # A05049) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
- Additional image
