F54316

antibody from NSJ Bioreagents

Targeting: SYP MRX96

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54316

Provider

NSJ Bioreagents

Product name

Synaptophysin Antibody / SYP

Antibody type

Polyclonal

Description

This highly specific Synaptophysin antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Synaptophysin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse 1) brain and 2) cerebellum lysate with Synaptophysin antibody. Predicted molecular weight: 34-38 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human brain tissue with Synaptophysin antibody (green) and DAPI nuclear stain (blue).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of mouse brain tissue with Synaptophysin antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human brain tissue with Synaptophysin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse hippocampus tissue with Synaptophysin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse Neuro-2a cells with Synaptophysin antibody; Blue=isotype control, Green= Synaptophysin antibody.