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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- Ab129952 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Synaptophysin Antibody
- Antibody type
- Recombinant
- Description
- Mouse anti Human Synaptophysin Antibody, Recombinant (MyM1-Syn), could be used for IHC and so on.ApplicationIHC: 1/100-1/200Protein FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- MyM1-Syn
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,48 mg/ml
- Storage
- Store at 2-8¡C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
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- Aladdin Scientific (provider)
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- Experimental details
- Recombinant Synaptophysin Antibody (Ab129952) - IHC Immunohistochemistry was performed on human pancreatic ductal carcinoma and human appendix tissue. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Following antigen retrieval, tissues were blocked in a 10% goat serum in wash buffer solution for 30 minutes at room temperature and endogenous peroxidase activity quenched with Peroxidase Suppressor. Tissue was then probed with a Recombinant Synaptophysin Antibody (Ab129952) at a dilution of 1:100 in 10% goat serum for overnight at 4℃. Using a Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) as secondary antibody, and detection was performed using a Streptavidin (HRP) (np156148) followed by colorimetric detection using DAB .Tissues were then counterstained with hematoxylin and prepped for mounting and imaging.
