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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- BS-2663R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLEC5A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen lysates probed with Rabbit Anti-CLEC5A Polyclonal Antibody, Unconjugated (bs-2663R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Antigen: bs-2663P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000; TMB staining; Read the data in Microplate Reader by 450nm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Antigen: bs-2663P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000; TMB staining; Read the data in Microplate Reader by 450nm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-CLEC5A/MDL1 Polyclonal Antibody (bs-2663R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U937 cells probed with Rabbit Anti-CLEC5A Polyclonal Antibody, FITC Conjugated (bs-2663R-FITC) at 1:50 for 40 minutes (green)compared to control cells (blue).
