MAB2384

antibody from R&D Systems

Targeting: CLEC5A CLECSF5, MDL-1

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2384

Provider

R&D Systems

Product name

Human MDL-1/CLEC5A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human MDL-1/CLEC5A in Western blots. Does not cross-react with recombinant mouse MDL-1.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q9NY25


Isotype

IgG

Antibody clone number

283834

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Atypical Activin A and IL-10 Production Impairs Human CD16+ Monocyte Differentiation into Anti-Inflammatory Macrophages.

González-Domínguez É, Domínguez-Soto Á, Nieto C, Flores-Sevilla JL, Pacheco-Blanco M, Campos-Peña V, Meraz-Ríos MA, Vega MA, Corbí ÁL, Sánchez-Torres C
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1327-37

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Cutting Edge: CLEC5A Mediates Macrophage Function and Chronic Obstructive Pulmonary Disease Pathologies.

Wortham BW, Eppert BL, Flury JL, Garcia SM, Donica WR, Osterburg A, Joyce-Shaikh B, Cua DJ, Borchers MT
Journal of immunology (Baltimore, Md. : 1950) 2016 Apr 15;196(8):3227-31

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Comparison of gene expression profiles between human and mouse monocyte subsets.

Ingersoll MA, Spanbroek R, Lottaz C, Gautier EL, Frankenberger M, Hoffmann R, Lang R, Haniffa M, Collin M, Tacke F, Habenicht AJ, Ziegler-Heitbrock L, Randolph GJ
Blood 2010 Jan 21;115(3):e10-9

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of MDL-1/CLEC5A in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human MDL-1/CLEC5A Monoclonal Antibody (Catalog # MAB2384, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).