MA5-25415

antibody from Invitrogen Antibodies

Targeting: UBL4A DXS254E, GDX, GET5, MDY2, TMA24, UBL4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-25415

Provider

Invitrogen Antibodies

Product name

UBL4A Monoclonal Antibody (OTI1D4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1D4

Vial size

100 µL

Concentration

0.52 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UBL4A in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MCF7 cells using 10 µg per lane. Samples were probed with UBL4A (Product # MA5-25415) monoclonal antibody at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UBL4A in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with UBL4A (Product # MA5-25415) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of UBL4A in COS7 cells. Cells were transfected with a plasmid overexpressing UBL4A and probed with a UBL4A monoclonal antibody (Product # MA5-25415).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a UBL4A monoclonal antibody (Product # MA5-25415).