PA5-27725

antibody from Invitrogen Antibodies

Targeting: GABBR1 GPRC3A, hGB1a

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27725

Provider

Invitrogen Antibodies

Product name

GABBR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: PC-3, U87-MG, SK-N-SH, C8D30.

Concentration

0.43 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GABBR1 Polyclonal Antibody detects GABBR1 protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract.7.5 % SDS-PAGE. GABBR1 Polyclonal Antibody (Product # PA5-27725) dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GABBR1 Polyclonal Antibody detects GABBR1 protein by Western blot analysis. A. 30 µg PC-3 whole cell lysate/extract. B. 30 µg U87-MG whole cell lysate/extract. C. 30 µg SK-N-SH whole cell lysate/extract.7.5 % SDS-PAGE. GABBR1 Polyclonal Antibody (Product # PA5-27725) dilution: 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GABBR1 was performed in SH-SY5Y cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABBR1 Polyclonal Antibody (Product # PA5-27725) diluted at 1:500. Red: SOX2 protein stained by SOX2 antibody. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GABBR1 was performed in U-87 MG cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABBR1 Polyclonal Antibody (Product # PA5-27725) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GABBR1 was performed in SH-SY5Y cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABBR1 Polyclonal Antibody (Product # PA5-27725) diluted at 1:500. Red: SOX2 protein stained by SOX2 antibody. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of GABBR1 was performed in paraffin-embedded rat brain tissue using GABBR1 Polyclonal Antibody (Product # PA5-27725) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GABBR1 Polyclonal Antibody detects GABA B Receptor 1 protein at cytosol on human brain by immunohistochemical analysis. Sample: Paraffin-embedded human brain. GABBR1 Polyclonal Antibody (Product # PA5-27725) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.