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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [28]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB1037-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Ly-6G/Ly-6C (Gr-1) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- RB6-8C5
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models.
PU.1 controls fibroblast polarization and tissue fibrosis.
Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis.
Vesicular nucleotide transporter mediates ATP release and migration in neutrophils.
Group 2 Innate Lymphoid Cells Attenuate Inflammatory Arthritis and Protect from Bone Destruction in Mice.
Colchicine alleviates acute postoperative pain but delays wound repair in mice: roles of neutrophils and macrophages.
Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice.
Monocytic and granulocytic myeloid derived suppressor cells differentially regulate spatiotemporal tumour plasticity during metastatic cascade.
Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33.
Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20.
Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation.
PTPN22 Is a Critical Regulator of Fcγ Receptor-Mediated Neutrophil Activation.
The Src-Family Kinases Hck and Fgr Regulate Early Lipopolysaccharide-Induced Myeloid Cell Recruitment into the Lung and Their Ability To Secrete Chemokines.
Rhadinovirus host entry by co-operative infection.
Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells.
Transcription factor binding site analysis identifies FOXO transcription factors as regulators of the cutaneous wound healing process.
Miro1 regulates intercellular mitochondrial transport & enhances mesenchymal stem cell rescue efficacy.
Pseudomonas aeruginosa MucD protease mediates keratitis by inhibiting neutrophil recruitment and promoting bacterial survival.
Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis.
Uropathogenic Escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide LL-37.
TLR4 activation is required for IL-17-induced multiple tissue inflammation and wasting in mice.
The leukotriene B4 receptor, BLT1, is required for the induction of experimental autoimmune encephalomyelitis.
Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
Syndecan-1 shedding facilitates the resolution of neutrophilic inflammation by removing sequestered CXC chemokines.
Myelomonocytic cell recruitment causes fatal CNS vascular injury during acute viral meningitis.
NOD1 expression in the eye and functional contribution to IL-1beta-dependent ocular inflammation in mice.
Kozasa K, Mabuchi S, Matsumoto Y, Kuroda H, Yokoi E, Komura N, Kawano M, Takahashi R, Sasano T, Shimura K, Kodama M, Hashimoto K, Sawada K, Nagasaka K, Kimura T
Oncotarget 2019 Mar 8;10(20):1887-1902
Oncotarget 2019 Mar 8;10(20):1887-1902
PU.1 controls fibroblast polarization and tissue fibrosis.
Wohlfahrt T, Rauber S, Uebe S, Luber M, Soare A, Ekici A, Weber S, Matei AE, Chen CW, Maier C, Karouzakis E, Kiener HP, Pachera E, Dees C, Beyer C, Daniel C, Gelse K, Kremer AE, Naschberger E, Stürzl M, Butter F, Sticherling M, Finotto S, Kreuter A, Kaplan MH, Jüngel A, Gay S, Nutt SL, Boykin DW, Poon GMK, Distler O, Schett G, Distler JHW, Ramming A
Nature 2019 Feb;566(7744):344-349
Nature 2019 Feb;566(7744):344-349
Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis.
Fujita Y, Khateb A, Li Y, Tinoco R, Zhang T, Bar-Yoseph H, Tam MA, Chowers Y, Sabo E, Gerassy-Vainberg S, Starosvetsky E, James B, Brown K, Shen-Orr SS, Bradley LM, Tessier PA, Ronai ZA
Cell reports 2018 Sep 18;24(12):3296-3311.e6
Cell reports 2018 Sep 18;24(12):3296-3311.e6
Vesicular nucleotide transporter mediates ATP release and migration in neutrophils.
Harada Y, Kato Y, Miyaji T, Omote H, Moriyama Y, Hiasa M
The Journal of biological chemistry 2018 Mar 9;293(10):3770-3779
The Journal of biological chemistry 2018 Mar 9;293(10):3770-3779
Group 2 Innate Lymphoid Cells Attenuate Inflammatory Arthritis and Protect from Bone Destruction in Mice.
Omata Y, Frech M, Primbs T, Lucas S, Andreev D, Scholtysek C, Sarter K, Kindermann M, Yeremenko N, Baeten DL, Andreas N, Kamradt T, Bozec A, Ramming A, Krönke G, Wirtz S, Schett G, Zaiss MM
Cell reports 2018 Jul 3;24(1):169-180
Cell reports 2018 Jul 3;24(1):169-180
Colchicine alleviates acute postoperative pain but delays wound repair in mice: roles of neutrophils and macrophages.
Sukeishi A, Isami K, Hiyama H, Imai S, Nagayasu K, Shirakawa H, Nakagawa T, Kaneko S
Molecular pain 2017 Jan-Dec;13:1744806917743680
Molecular pain 2017 Jan-Dec;13:1744806917743680
Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice.
Busch CJ, Hendrikx T, Weismann D, Jäckel S, Walenbergh SM, Rendeiro AF, Weißer J, Puhm F, Hladik A, Göderle L, Papac-Milicevic N, Haas G, Millischer V, Subramaniam S, Knapp S, Bennett KL, Bock C, Reinhardt C, Shiri-Sverdlov R, Binder CJ
Hepatology (Baltimore, Md.) 2017 Apr;65(4):1181-1195
Hepatology (Baltimore, Md.) 2017 Apr;65(4):1181-1195
Monocytic and granulocytic myeloid derived suppressor cells differentially regulate spatiotemporal tumour plasticity during metastatic cascade.
Ouzounova M, Lee E, Piranlioglu R, El Andaloussi A, Kolhe R, Demirci MF, Marasco D, Asm I, Chadli A, Hassan KA, Thangaraju M, Zhou G, Arbab AS, Cowell JK, Korkaya H
Nature communications 2017 Apr 6;8:14979
Nature communications 2017 Apr 6;8:14979
Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33.
Saranchova I, Han J, Huang H, Fenninger F, Choi KB, Munro L, Pfeifer C, Welch I, Wyatt AW, Fazli L, Gleave ME, Jefferies WA
Scientific reports 2016 Sep 13;6:30555
Scientific reports 2016 Sep 13;6:30555
Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20.
Finley PJ, DeClue CE, Sell SA, DeBartolo JM, Shornick LP
Advances in wound care 2016 Nov 1;5(11):486-494
Advances in wound care 2016 Nov 1;5(11):486-494
Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation.
Ashraf MI, Schwelberger HG, Brendel KA, Feurle J, Andrassy J, Kotsch K, Regele H, Pratschke J, Maier HT, Aigner F
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2016 Mar;16(3):808-20
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2016 Mar;16(3):808-20
PTPN22 Is a Critical Regulator of Fcγ Receptor-Mediated Neutrophil Activation.
Vermeren S, Miles K, Chu JY, Salter D, Zamoyska R, Gray M
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 15;197(12):4771-4779
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 15;197(12):4771-4779
The Src-Family Kinases Hck and Fgr Regulate Early Lipopolysaccharide-Induced Myeloid Cell Recruitment into the Lung and Their Ability To Secrete Chemokines.
Mazzi P, Caveggion E, Lapinet-Vera JA, Lowell CA, Berton G
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2383-95
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2383-95
Rhadinovirus host entry by co-operative infection.
Lawler C, Milho R, May JS, Stevenson PG
PLoS pathogens 2015 Mar;11(3):e1004761
PLoS pathogens 2015 Mar;11(3):e1004761
Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
Kowalewska PM, Patrick AL, Fox-Robichaud AE
PloS one 2014;9(9):e104537
PloS one 2014;9(9):e104537
CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells.
Lewis ND, Muthukumarana A, Fogal SE, Corradini L, Stefanopoulos DE, Adusumalli P, Pelletier J, Panzenbeck M, Berg K, Canfield M, Cook BN, Razavi H, Kuzmich D, Anderson S, Allard D, Harrison P, Grimaldi C, Souza D, Harcken C, Fryer RM, Modis LK, Brown ML
PloS one 2014;9(8):e105883
PloS one 2014;9(8):e105883
Transcription factor binding site analysis identifies FOXO transcription factors as regulators of the cutaneous wound healing process.
Roupé KM, Veerla S, Olson J, Stone EL, Sørensen OE, Hedrick SM, Nizet V
PloS one 2014;9(2):e89274
PloS one 2014;9(2):e89274
Miro1 regulates intercellular mitochondrial transport & enhances mesenchymal stem cell rescue efficacy.
Ahmad T, Mukherjee S, Pattnaik B, Kumar M, Singh S, Kumar M, Rehman R, Tiwari BK, Jha KA, Barhanpurkar AP, Wani MR, Roy SS, Mabalirajan U, Ghosh B, Agrawal A
The EMBO journal 2014 May 2;33(9):994-1010
The EMBO journal 2014 May 2;33(9):994-1010
Pseudomonas aeruginosa MucD protease mediates keratitis by inhibiting neutrophil recruitment and promoting bacterial survival.
Mochizuki Y, Suzuki T, Oka N, Zhang Y, Hayashi Y, Hayashi N, Gotoh N, Ohashi Y
Investigative ophthalmology & visual science 2014 Jan 9;55(1):240-6
Investigative ophthalmology & visual science 2014 Jan 9;55(1):240-6
Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
Tashiro Y, Nishida C, Sato-Kusubata K, Ohki-Koizumi M, Ishihara M, Sato A, Gritli I, Komiyama H, Sato Y, Dan T, Miyata T, Okumura K, Tomiki Y, Sakamoto K, Nakauchi H, Heissig B, Hattori K
Blood 2012 Jun 28;119(26):6382-93
Blood 2012 Jun 28;119(26):6382-93
CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis.
Ishii M, Asano K, Namkoong H, Tasaka S, Mizoguchi K, Asami T, Kamata H, Kimizuka Y, Fujiwara H, Funatsu Y, Kagawa S, Miyata J, Ishii K, Nakamura M, Hirai H, Nagata K, Kunkel SL, Hasegawa N, Betsuyaku T
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 1;188(11):5655-64
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 1;188(11):5655-64
Uropathogenic Escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide LL-37.
Kai-Larsen Y, Lüthje P, Chromek M, Peters V, Wang X, Holm A, Kádas L, Hedlund KO, Johansson J, Chapman MR, Jacobson SH, Römling U, Agerberth B, Brauner A
PLoS pathogens 2010 Jul 22;6(7):e1001010
PLoS pathogens 2010 Jul 22;6(7):e1001010
TLR4 activation is required for IL-17-induced multiple tissue inflammation and wasting in mice.
Tang H, Pang S, Wang M, Xiao X, Rong Y, Wang H, Zang YQ
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2563-9
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2563-9
The leukotriene B4 receptor, BLT1, is required for the induction of experimental autoimmune encephalomyelitis.
Kihara Y, Yokomizo T, Kunita A, Morishita Y, Fukayama M, Ishii S, Shimizu T
Biochemical and biophysical research communications 2010 Apr 9;394(3):673-8
Biochemical and biophysical research communications 2010 Apr 9;394(3):673-8
Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
International journal of cancer 2009 Sep 15;125(6):1276-84
International journal of cancer 2009 Sep 15;125(6):1276-84
Syndecan-1 shedding facilitates the resolution of neutrophilic inflammation by removing sequestered CXC chemokines.
Hayashida K, Parks WC, Park PW
Blood 2009 Oct 1;114(14):3033-43
Blood 2009 Oct 1;114(14):3033-43
Myelomonocytic cell recruitment causes fatal CNS vascular injury during acute viral meningitis.
Kim JV, Kang SS, Dustin ML, McGavern DB
Nature 2009 Jan 8;457(7226):191-5
Nature 2009 Jan 8;457(7226):191-5
NOD1 expression in the eye and functional contribution to IL-1beta-dependent ocular inflammation in mice.
Rosenzweig HL, Galster KT, Planck SR, Rosenbaum JT
Investigative ophthalmology & visual science 2009 Apr;50(4):1746-53
Investigative ophthalmology & visual science 2009 Apr;50(4):1746-53
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ly-6G/Ly-6C (Gr-1) in Mouse Spleno-cytes. Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed mouse splenocytes using Mouse Ly-6G/Ly-6C (Gr-1) Monoclonal Anti-body (Catalog # MAB1037) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ly-6G/Ly-6C (Gr-1) in Mouse Spleen. Ly-6G/Ly-6C (Gr-1) was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Monoclonal Antibody (Catalog # MAB1037) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
