AF6948

antibody from Novus Biologicals

Targeting: CLTCL1 CHC22, CLH22, CLTCL, CLTD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF6948

Provider

Novus Biologicals

Product name

Sheep Polyclonal Clathrin Heavy Chain 2/CHC22/CLTCL1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Clathrin Heavy Chain 2/CHC22 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC17 is observed.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development.

Nahorski MS, Borner GHH, Shaikh SS, Davies AK, Al-Gazali L, Antrobus R, Woods CG
Scientific reports 2018 Feb 5;8(1):2340

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Clathrin Heavy Chain 2/CHC22 by Western Blot. Western blot shows lysates of human testis tissue and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Clathrin Heavy Chain 2/CHC22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Clathrin Heavy Chain 2/CHC22 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.