Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6948 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Clathrin Heavy Chain 2/CHC22/CLTCL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Clathrin Heavy Chain 2/CHC22 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC17 is observed.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development.
Nahorski MS, Borner GHH, Shaikh SS, Davies AK, Al-Gazali L, Antrobus R, Woods CG
Scientific reports 2018 Feb 5;8(1):2340
Scientific reports 2018 Feb 5;8(1):2340
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Clathrin Heavy Chain 2/CHC22 by Western Blot. Western blot shows lysates of human testis tissue and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Clathrin Heavy Chain 2/CHC22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Clathrin Heavy Chain 2/CHC22 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.