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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-25804 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Clathrin Heavy Chain Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references ROCK1 is a novel Rac1 effector to regulate tubular endocytic membrane formation during clathrin-independent endocytosis.
Soriano-Castell D, Chavero A, Rentero C, Bosch M, Vidal-Quadras M, Pol A, Enrich C, Tebar F
Scientific reports 2017 Jul 31;7(1):6866
Scientific reports 2017 Jul 31;7(1):6866
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a CLTC polyclonal antibody (Product # PA5-25804) in HeLa cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human brain tissue using a CLTC polyclonal antibody (Product # PA5-25804), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HeLa cells using a CLTC polyclonal antibody (Product # PA5-25804) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 W13-induced PM tubules provide an internalization pathway for beta1-integrin. ( a ) COS1 cells grown on coverslips expressing the membrane marker GFP-mem were incubated with an anti-beta1-integrin rat antibody for 30 minutes at 4 degC to avoid endocytosis, followed by incubation for 10 minutes at 37 degC to allow internalization in the presence of W13 (20 min, 4.5 ug/ml). After fixation, beta1-integrin was detected with an Alexa-555 labeled anti-rat antibody, and images were acquired with a confocal microscope (Leica TCS SP5). The higher magnification insets show beta1-integrin localization in W13-induced tubules (green arrowheads). ( b , c ) Following the same procedure explained as in ( a ), beta1-integrin was detected with an Alexa-647 labeled anti-rat antibody and EEA1 with a specific antibody and the secondary Alexa-555 anti-mouse in untreated ( b ) or W13-treated cells ( c ). Insets show beta1-integrin in EEA1-positive endosomes (red arrowheads) ( b ) or in tubules (green arrowheads) ( c ) (bars, 10 um). ( d , e ) Quantification of internalized beta1-integrin ( d ) and transferrin ( e ), as explained in the Materials and Methods , in COS1 cells expressing GFP-mem or GFP-Rac1 G12V for the indicated conditions (W13t, cells presenting tubules; W13nt, cells without tubules). Mean values +- standard error of the mean (SEM) from three independent experiments are shown. Statistical significance between different conditions and the corresponding control was determined
