Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF5024 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CD99-L2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CD99-L2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human CD99-L2 and recombinant mouse CD99 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Interaction of CD99 with its paralog CD99L2 positively regulates CD99L2 trafficking to cell surfaces.
Nam G, Lee YK, Lee HY, Ma MJ, Araki M, Araki K, Lee S, Lee IS, Choi EY
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5730-42
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5730-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CD99-L2 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse CD99-L2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5024, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).